ITGB5 as a Potential Diagnostic Biomarker for Osteoarthritis.

IF 1.7 4区医学 Q4 NEUROSCIENCES

Journal of musculoskeletal & neuronal interactions Pub Date : 2025-03-01 DOI:10.22540/JMNI-25-086

Shouchao Hu, Jianxin Huang, Wenzhi Wang

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引用次数: 0

Abstract

Objective: Osteoarthritis (OA) is a prevalent degenerative joint disease, especially occur in the elderly. This study aimed to uncover a novel biomarker for early diagnosis and treatment of OA.

Methods: WGCNA, differential expression analysis and PPI network were used for screening hub genes-related to OA, utilizing the GSE55235 and GSE57218 datasets from GEO database.

Results: Based on the data in the GEO datasets, compared to normal tissues, ITGB5 was obviously elevated in OA cartilage and synovial samples. Additionally, ROC curve results validated the diagnostic value of ITGB5 in OA. Mechanistically, transcription factors KLF4 and KLF11 could modulate ITGB5 gene transcription via binding to its promoter region, thereby affecting ITGB5 gene expression in OA tissues. GSEA results showed that ITGB5 gene was closely related to p53, wnt, TNF and T cell receptor signaling pathways, suggesting that ITGB5 may play potential roles in affecting cell apoptosis and inflammation in OA. Moreover, ITGB5 levels in OA samples was positively correlated to T helper type 1 cells, natural killer T cells, macrophages, memory CD8 T cells, activated dendritic cells.

Conclusion: In this study, we found that ITGB5 was obviously elevated in OA samples. Moreover, ITGB5 may function as a diagnostic biomarker in OA.

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ITGB5 作为骨关节炎的潜在诊断生物标志物

目的：骨关节炎（OA）是一种常见的退行性关节疾病，多见于老年人。本研究旨在发现一种新的早期诊断和治疗OA的生物标志物。方法：利用GEO数据库的GSE55235和GSE57218数据集，采用WGCNA、差异表达分析和PPI网络筛选OA相关枢纽基因。结果：基于GEO数据集的数据，与正常组织相比，OA软骨和滑膜样本中ITGB5明显升高。ROC曲线结果验证了ITGB5对OA的诊断价值。机制上，转录因子KLF4和KLF11通过结合ITGB5基因启动子区调控ITGB5基因的转录，从而影响OA组织中ITGB5基因的表达。GSEA结果显示，ITGB5基因与p53、wnt、TNF和T细胞受体信号通路密切相关，提示ITGB5可能在影响OA细胞凋亡和炎症中发挥潜在作用。此外，OA样品中ITGB5水平与辅助性T型1细胞、自然杀伤T细胞、巨噬细胞、记忆性CD8 T细胞、活化的树突状细胞呈正相关。结论：在本研究中，我们发现OA样本中ITGB5明显升高。此外，ITGB5可能作为OA的诊断性生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of musculoskeletal & neuronal interactions NEUROSCIENCES-PHYSIOLOGY

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Musculoskeletal and Neuronal Interactions (JMNI) is an academic journal dealing with the pathophysiology and treatment of musculoskeletal disorders. It is published quarterly (months of issue March, June, September, December). Its purpose is to publish original, peer-reviewed papers of research and clinical experience in all areas of the musculoskeletal system and its interactions with the nervous system, especially metabolic bone diseases, with particular emphasis on osteoporosis. Additionally, JMNI publishes the Abstracts from the biannual meetings of the International Society of Musculoskeletal and Neuronal Interactions, and hosts Abstracts of other meetings on topics related to the aims and scope of JMNI.