Association of TNFRSF13B Gene Polymorphisms With SARS-CoV-2 Infection, Severity, and Humoral Immune Response in a Moroccan Population

IF 2.3 4区 医学 Q3 GENETICS & HEREDITY
Oumaima Laazaazia, Ahd Ouladlahsen, Safaa Aqillouch, Haya Altawalah, Oumaima Bouddahab, Rachid Noureddine, Pascal Pineau, Mustapha Lkhider, Sayeh Ezzikouri
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引用次数: 0

Abstract

Background and Aims: Genetic factors, including polymorphisms in the TNFRSF13B gene, which regulates humoral immunity, can influence susceptibility to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This study aims to investigate the association between two polymorphisms, rs12603708 and rs3751987, and SARS-CoV-2 susceptibility, disease severity, and humoral immune responses in a Moroccan population.

Materials and Methods: A total of 303 unvaccinated COVID-19 patients (151 severe cases and 152 asymptomatic/moderate cases) and 150 individuals from a SARS-CoV-2-negative group were included in the analysis. Genotyping was performed using TaqMan SNP assays. SARS-CoV-2 antibodies targeting the nucleocapsid protein and IgG antibodies specific to the receptor-binding domain (RBD) were quantified using chemiluminescence microparticles immunoassay. Complete blood counts and C-reactive protein levels were evaluated using an automated platform.

Results: Our analysis revealed that the A/A genotype of rs12603708 significantly increased the risk of SARS-CoV-2 infection in both codominant (p = 0.0055; OR = 3.74; adjusted p value = 0.022) and recessive (p = 0.0049; OR = 3.17; adjusted p value = 0.022) models, as well as the risk of severe disease (p = 0.014; OR = 3.43; adjusted p value = 0.049). For rs3751987, the G/G genotype was linked to higher susceptibility to infection (p = 0.0011; OR = 2.91; adjusted p value = 0.008), while the G/A genotype appeared protective (p = 0.0007; OR = 0.45; adjusted p value = 0.008). No association was found between rs3751987 and disease severity. Analysis of IgG anti-N and anti-RBD levels revealed no significant associations with either polymorphism (p > 0.05).

Conclusion: These findings highlight the role of TNFRSF13B polymorphisms in SARS-CoV-2 susceptibility and severity, while their impact on humoral immune responses appears limited.

摩洛哥人群中TNFRSF13B基因多态性与SARS-CoV-2感染、严重程度和体液免疫反应的关系
背景与目的:调节体液免疫的TNFRSF13B基因多态性等遗传因素可能影响对严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)的易感性。本研究旨在调查摩洛哥人群中rs12603708和rs3751987两种多态性与SARS-CoV-2易感性、疾病严重程度和体液免疫反应之间的关系。材料与方法:共纳入未接种疫苗的COVID-19患者303例(重症151例,无症状/中度152例)和sars - cov -2阴性组150例。采用TaqMan SNP检测进行基因分型。采用化学发光微粒子免疫分析法对靶向核衣壳蛋白的SARS-CoV-2抗体和受体结合域(RBD)特异性IgG抗体进行定量分析。使用自动化平台评估全血细胞计数和c反应蛋白水平。结果:我们的分析显示,rs12603708的A/A基因型显著增加了两种共显性人群感染SARS-CoV-2的风险(p = 0.0055;Or = 3.74;校正p值= 0.022)和隐性(p = 0.0049;Or = 3.17;调整后的p值= 0.022)模型,以及严重疾病的风险(p = 0.014;Or = 3.43;调整p值= 0.049)。对于rs3751987, G/G基因型与较高的感染易感性相关(p = 0.0011;Or = 2.91;调整p值= 0.008),而G/A基因型具有保护作用(p = 0.0007;Or = 0.45;调整p值= 0.008)。rs3751987与疾病严重程度无关联。IgG抗n和抗rbd水平分析显示,两种多态性均无显著相关性(p < 0.05)。结论:这些发现突出了TNFRSF13B多态性在SARS-CoV-2易感性和严重程度中的作用,而它们对体液免疫反应的影响似乎有限。
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来源期刊
CiteScore
4.70
自引率
0.00%
发文量
48
审稿时长
6-12 weeks
期刊介绍: The International Journal of Immunogenetics (formerly European Journal of Immunogenetics) publishes original contributions on the genetic control of components of the immune system and their interactions in both humans and experimental animals. The term ''genetic'' is taken in its broadest sense to include studies at the evolutionary, molecular, chromosomal functional and population levels in both health and disease. Examples are: -studies of blood groups and other surface antigens- cell interactions and immune response- receptors, antibodies, complement components and cytokines- polymorphism- evolution of the organisation, control and function of immune system components- anthropology and disease associations- the genetics of immune-related disease: allergy, autoimmunity, immunodeficiency and other immune pathologies- All papers are seen by at least two independent referees and only papers of the highest quality are accepted.
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