Cyto-genotoxic effects predict ecotoxicity in plant bioassays and the aquatic organism Artemia salina L.: a case study from a sewage treatment plant.

IF 1.9 4区 环境科学与生态学 Q4 ENGINEERING, ENVIRONMENTAL
Marcela Emiliano Novaes Matilde, Leonardo Mendes da Silva, Tamara Alessandra Costa Santos, Maria Eduarda Magalhães, Marcel José Palmieri, Larissa Fonseca Andrade-Vieira
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Abstract

This study evaluated the toxicological and mutagenic potential of water samples from a Wastewater Treatment Plant (WWTP) in Lavras, Minas Gerais, Brazil. Samples were taken from four sites: upstream in the stream (P1), downstream (P2), at the entrance of the treatment station (P3), and at the exit (P4). We conducted physicochemical analyses in water, phytotoxicity tests on plants (Triticum aestivum, Pennisetum glaucum, Lactuca sativa, Raphanus sativus), cytogenotoxicity tests using onion roots (Allium cepa), and Artemia salina immobilization tests. Elevated Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), anionic surfactants, and ammoniacal nitrogen were found at P3 and P4. While germination rates were generally unaffected, P4 inhibited the germination speed of R. sativus. The growth of L. sativa increased in P3 and P4, and of R. sativus in P2, due to more nitrogen and phosphorus. T. aestivum and P. glaucum, however, had their growth inhibited at P4 due to surfactant toxicity. Cytogenotoxicity tests revealed the highest frequencies of micronuclei and nuclear buds in cells exposed to P3 and P4. Additionally, P3 caused 87.5% immobilization of A. salina. These findings suggest that the WWTP is not fully efficient, and its effluent discharge may contribute to eutrophication and genetic mutations in exposed organisms.

细胞-基因毒性效应在植物生物测定和水生生物中预测生态毒性:来自污水处理厂的案例研究。
本研究评估了巴西米纳斯吉拉斯州拉夫拉斯市污水处理厂(WWTP)水样的毒理学和诱变潜力。样本取自四个地点:河流上游(P1)、下游(P2)、处理站入口(P3)和出口(P4)。我们在水中进行了理化分析,对植物(Triticum aestivum, Pennisetum glaucum, lacuca sativa, Raphanus sativus)进行了植物毒性试验,用洋葱根(Allium cepa)进行了细胞毒性试验,并对Artemia salina进行了固定化试验。P3和P4的生化需氧量(BOD)、化学需氧量(COD)、阴离子表面活性剂和氨态氮均升高。P4抑制了红花的萌发速度,但对萌发率没有影响。由于氮磷含量较高,P3、P4和P2的油菜生长加快。而T. aestivum和P. glaucum的生长在P4时受到表面活性剂毒性的抑制。细胞基因毒性试验显示,暴露于P3和P4的细胞中微核和核芽的频率最高。P3对褐藻的固定作用为87.5%。这些发现表明,污水处理厂并非完全有效,其污水排放可能导致暴露生物的富营养化和基因突变。
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来源期刊
CiteScore
4.10
自引率
4.80%
发文量
93
审稿时长
3.0 months
期刊介绍: 14 issues per year Abstracted/indexed in: BioSciences Information Service of Biological Abstracts (BIOSIS), CAB ABSTRACTS, CEABA, Chemical Abstracts & Chemical Safety NewsBase, Current Contents/Agriculture, Biology, and Environmental Sciences, Elsevier BIOBASE/Current Awareness in Biological Sciences, EMBASE/Excerpta Medica, Engineering Index/COMPENDEX PLUS, Environment Abstracts, Environmental Periodicals Bibliography & INIST-Pascal/CNRS, National Agriculture Library-AGRICOLA, NIOSHTIC & Pollution Abstracts, PubSCIENCE, Reference Update, Research Alert & Science Citation Index Expanded (SCIE), Water Resources Abstracts and Index Medicus/MEDLINE.
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