Control of microbial contamination in dental unit waterlines: Effectiveness of neutral electrolytic water

Abstract

Objective

With an emphasis on nosocomial infection control, dental healthcare settings face challenges in managing and preventing microbial contamination, particularly in dental unit waterlines (DUWL). Currently, there are a lack of studies on clear measures to prevent contamination of DUWLs. Therefore, in this study, we investigated the effectiveness of neutral electrolytic water in controlling microbial contamination of DUWLs.

Methods

Water samples collected from DUWL were tested for Legionella species. Adenosine triphosphate levels were measured as indicators of contamination in the DUWL and water supply models. Heterotrophic bacterial counts were assessed before and after implementation of neutral electrolytic water purification systems. During dental treatment, plate sampling of heterotrophic bacteria was conducted before and after implementation of the neutral electrolytic water purification systems.

Results

Legionella spp. were not detected in the water samples collected from DUWL. However, samples collected using three-way syringes, gargle water, and high-speed handpieces indicated inadequate hygiene levels. Heterotrophic bacterial counts were significantly reduced after neutral electrolytic water purification systems were implemented. In the environmental survey conducted during dental treatment, no significant differences were found between tap water and neutral electrolytic water in terms of heterotrophic bacterial counts.

Conclusions

Our results demonstrate the effectiveness of neutral electrolytic water in reducing microbial contamination in DUWLs. Additionally, use of an extraoral vacuum aspirator during dental procedures can significantly reduce the risk of infection. These findings highlight the importance of implementing effective disinfection strategies to ensure patient safety in dental settings.