Genome-based taxonomy of the family Haloarculaceae, proposal of Natronomonadaceae fam. nov., and description of four novel halophilic archaea from two saline lakes and a marine solar saltern

IF 3.3 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Systematic and applied microbiology Pub Date : 2025-02-27 DOI:10.1016/j.syapm.2025.126592

Ling-Rui Zhu, Ya-Ling Mao, Yao Hu, Ya-Ping Sun, Jing Hou, Heng-Lin Cui

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引用次数: 0

Abstract

A new family related to the family Haloarculaceae was proposed and the genus Actinarchaeum was merged into the genus Halocatena through phylogenetic, phylogenomic, and comparative genomic analyses. Four strains KK48^T, YCN56^T, SYNS191^T, and SYNS196^T with new taxonomic status were isolated from inland saline lakes and a marine solar saltern. According to the comparison of 16S rRNA gene and rpoB' gene sequences, strains KK48^T, YCN56^T, SYNS191^T, and SYNS196^T showed high sequence similarities to the genera Salinibaculum and Salinirubellus, respectively. The values of average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity between these strains and the species of Salinibaculum and Salinirubellus ranged from 75.3 to 77.7 %, 24.5–25.9 % and 66.3–73.4 %, respectively. These data were well below the threshold for species classification, supporting their placements in new taxa. The major polar lipids of these strains were phosphatidic acid, phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, sulfated mannosyl glucosyl diether, mannosyl glucosyl diether, and disulfated mannosyl glucosyl diether. Based on the phenotypic, chemotaxonomic, phylogenetic, and phylogenomic properties, strains KK48^T (= CGMCC 1.19060^T = JCM 35607^T), YCN56^T (= CGMCC 1.62603^T = JCM 36493^T), SYNS191^T (= CGMCC 1.62607^T = JCM 36494^T), and SYNS196^T (= CGMCC 1.62608^T = JCM 36495^T) represent four novel species of the genera Salinibaculum and Salinirubellus. And Salinibaculum rarum sp. nov., Salinibaculum salinum sp. nov., Salinibaculum marinum sp. nov., and Salinirubellus litoreus sp. nov. are proposed to accommodate these strains.

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通过系统发生学、系统基因组学和比较基因组学分析，提出了一个与 Haloarculaceae 科相关的新科，并将 Actinarchaeum 属并入 Halocatena 属。从内陆盐湖和海洋日光浴盐场分离出四株具有新分类地位的菌株 KK48T、YCN56T、SYNS191T 和 SYNS196T。根据 16S rRNA 基因和 rpoB'基因序列的比较，KK48T、YCN56T、SYNS191T 和 SYNS196T 菌株分别与 Salinibaculum 属和 Salinirubellus 属具有高度的序列相似性。这些菌株与 Salinibaculum 属和 Salinirubellus 属的平均核苷酸同一性、数字 DNA-DNA 杂交和平均氨基酸同一性值分别为 75.3%-77.7%、24.5%-25.9% 和 66.3%-73.4%。这些数据远低于物种分类的临界值，支持将它们归入新类群。这些菌株的主要极性脂质为磷脂酸、磷脂酰甘油、磷脂酰甘油磷酸甲酯、硫酸磷脂酰甘油、硫酸化甘露糖基二醚、甘露糖基二醚和二硫化甘露糖基二醚。根据表型、化学分类学、系统发育和系统基因组特性，菌株 KK48T（= CGMCC 1.19060T = JCM 35607T）、YCN56T（= CGMCC 1.62603T = JCM 36493T）、SYNS191T（= CGMCC 1.62607T = JCM 36494T）和 SYNS196T（= CGMCC 1.62608T = JCM 36495T）代表 Salinibaculum 属和 Salinirubellus 属的四个新种。新种 Salinibaculum rarum sp.nov.、新种 Salinibaculum salinum sp.nov.、新种 Salinibaculum marinum sp.nov.和新种 Salinirubellus litoreus sp.

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来源期刊

Systematic and applied microbiology 生物-生物工程与应用微生物

CiteScore

7.50

自引率

5.90%

发文量

审稿时长

22 days

期刊介绍： Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: