HmGST9, an anthocyanin-related glutathione S-transferase gene, is essential for sepals coloration in Hydrangea macrophylla

IF 4 3区生物学 Q1 PLANT SCIENCES

Journal of plant physiology Pub Date : 2025-02-25 DOI:10.1016/j.jplph.2025.154466

Haixia Chen, Penghu Lei, Huijun Zhang, Yajing Wang, Xuan Li, Hui Jiang, Jiren Chen

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引用次数: 0

Abstract

Flower color is an important ornamental trait of Hydrangea macrophylla. The transport of anthocyanin from endoplasmic reticulum to vacuole for storage is the basis of flower color formation. Glutathione S-transferase (GST) plays an important role in this transport process. However, little is known about the GST genes involved in anthocyanin transport and their functions in H. macrophylla. In this study, the sepals of H. macrophylla ‘Blue Mom’ was used as the experimental material. The gene HmGST9 that may be involved in anthocyanin accumulation was identified from the genome, and it was found to be located in the endoplasmic reticulum and tonoplast. Through Arabidopsis tt19 mutant molecular complementation experiment, it was proved that HmGST9 could restore anthocyanin accumulation in vegetative tissues of Arabidopsis tt19 mutant, but could not restore the color of seed coat. In the sepals of H. macrophylla, virus-induced HmGST9 gene silencing resulted in a significant decrease in anthocyanin content, and the structural genes and transcription factors in the anthocyanin biosynthesis pathway were also significantly down-regulated. In contrast, transient overexpression of HmGST9 in sepals resulted in a significant increase in anthocyanin content and promoted the up-regulation of related structural genes and transport genes. These results indicate that HmGST9 plays an important role in regulating the transport and accumulation of anthocyanins in H. macrophylla, and is of great significance for analyzing the molecular mechanism of flower color formation in H. macrophylla.

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来源期刊

Journal of plant physiology 生物-植物科学

CiteScore

7.20

自引率

4.70%

发文量

196

审稿时长

32 days

期刊介绍： The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.