Evolutional, expressional and functional analysis of WRKY gene family reveals that PbeWRKY16 and PbeWRKY31 contribute to the Valsa canker resistance in Pyrus betulifolia

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-02-28 DOI:10.1016/j.plaphy.2025.109719

Chenglong Du , Hongqiang Yu , Huanhuan Hu , E. Sun , Minrui Cai , Zhiqi Dou , Han Dong , Cunwu Zuo

{"title":"Evolutional, expressional and functional analysis of WRKY gene family reveals that PbeWRKY16 and PbeWRKY31 contribute to the Valsa canker resistance in Pyrus betulifolia","authors":"Chenglong Du , Hongqiang Yu , Huanhuan Hu , E. Sun , Minrui Cai , Zhiqi Dou , Han Dong , Cunwu Zuo","doi":"10.1016/j.plaphy.2025.109719","DOIUrl":null,"url":null,"abstract":"<div><div>The WRKY transcription factor family plays a crucial role in regulating plant growth and stress responses. However, there are few studies on the regulation of resistance to Valsa canker. In this study, a comprehensive analysis of WRKY genes across 19 plant species was conducted. The potential members of Valsa canker resistance regulation were identified via functional validation. A total of 1641 WRKY genes could be categorized into seven groups. WRKY family members show subfamily- and species-specific expansions. In Rosaceae, Group II-d and II-e were rapidly expanded, which mainly originated based from whole genome duplication (WGD). Cis-element analysis and protein interaction network prediction underscored that most WRKYs respond to stress signals. Based on expressional investigation and Weighted Gene Co-expression Network Analysis (WGCNA), 9 WRKY genes in <em>Pyrus betulaefolia</em> were screened as candidates for Valsa canker resistance regulation. Functional analysis further demonstrated that PbeWRKY16 and PbeWRKY31 regulate the expression of genes involved in salicylic acid (SA) biosynthesis and transport, thereby enhancing resistance to Valsa canker and activating immune responses. Our results provide a foundation for understanding the evolutionary mechanisms of the WRKY gene family and screened potential family members on Valsa canker resistance regulation.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"222 ","pages":"Article 109719"},"PeriodicalIF":6.1000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825002475","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

The WRKY transcription factor family plays a crucial role in regulating plant growth and stress responses. However, there are few studies on the regulation of resistance to Valsa canker. In this study, a comprehensive analysis of WRKY genes across 19 plant species was conducted. The potential members of Valsa canker resistance regulation were identified via functional validation. A total of 1641 WRKY genes could be categorized into seven groups. WRKY family members show subfamily- and species-specific expansions. In Rosaceae, Group II-d and II-e were rapidly expanded, which mainly originated based from whole genome duplication (WGD). Cis-element analysis and protein interaction network prediction underscored that most WRKYs respond to stress signals. Based on expressional investigation and Weighted Gene Co-expression Network Analysis (WGCNA), 9 WRKY genes in Pyrus betulaefolia were screened as candidates for Valsa canker resistance regulation. Functional analysis further demonstrated that PbeWRKY16 and PbeWRKY31 regulate the expression of genes involved in salicylic acid (SA) biosynthesis and transport, thereby enhancing resistance to Valsa canker and activating immune responses. Our results provide a foundation for understanding the evolutionary mechanisms of the WRKY gene family and screened potential family members on Valsa canker resistance regulation.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.