Lenu B. Fakae, Jizhou Zhong, Ka Lung Andrew Chan, Subbareddy Mekapothula, Gareth W. V. Cave, Xing-Quan Zhu, Carl W. Stevenson, Hany M. Elsheikha
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引用次数: 0
Abstract
Purpose
Acanthamoeba castellanii is an important opportunistic human protozoal pathogen that can cause both skin, ocular and brain infections. Recent studies have established that brews and solvent extract (SE) of green tea (Camellia sinensis) can inhibit the growth and encystation of A. castellanii. Here we characterized those growth and encystation inhibitions.
Methods
Herein, we characterize of the morphological and chemical changes that occur in the trophozoites and the encysting stage of A. castellanii after exposure to C. sinensis SE and brew using Transmission electron microscopy (TEM), Fourier-transform infrared (FTIR) microspectroscopy and fluorescence-based assays.
Results
TEM showed ultrastructural changes in both A. castellanii stages. FTIR microspectroscopy revealed modifications of amide I and II band peaks in the C. sinensis-treated trophozoites, suggesting an inhibition of protein synthesis. Assessment of the nucleus integrity of trophozoites exposed to SE and brew revealed disruption of the nuclear membrane integrity, nuclear fragmentation, and chromatin degradation, and reduction in the quantity of DNA and RNA, indicating trophozoite death. These results are consistent with C. sinensis acting as a membrane-active anti-acanthamoebic, exhibiting amoebicidal activity against growing and encysting A. castellanii. This work underlines the importance of characterizing the effect of C. sinensis constituents, individually or in combinations, to clarify which ones are the primary components responsible for its action and the observed alterations in the structure and function of A. castellanii.
Conclusion
These results demonstrated that exposure to C. sinensis SE or brew alters the synthesis of protein, DNA, RNA and disrupts the cell wall integrity.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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