Dual-crosslinked zwitterionic hydrogel: a facile platform of wearable colorimetric urea sensors

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-03-04 DOI:10.1007/s00604-025-07060-w

Benjawan Somchob, Nichaphat Passornraprasit, Voravee P. Hoven, Nadnudda Rodthongkum

{"title":"Dual-crosslinked zwitterionic hydrogel: a facile platform of wearable colorimetric urea sensors","authors":"Benjawan Somchob, Nichaphat Passornraprasit, Voravee P. Hoven, Nadnudda Rodthongkum","doi":"10.1007/s00604-025-07060-w","DOIUrl":null,"url":null,"abstract":"<div><p>Zwitterionic hydrogel based on a dual-crosslinked network of pluronic F-127 dimethacrylate (PLU-DMA) and a terpolymer, poly(sulfobetaine methacrylate)-<i>co</i>-methacrylic acid-<i>co</i>–<i>N</i>-methacryloyloxyethyl tyrosine methylester) (PSBMM) was prepared and successfully applied as an enzyme-based colorimetric sensor of urea on diaper. The prepared hydrogel possessed good mechanical property while preserving its swelling capability. The urease-incorporated hydrogel exhibited a vivid color change from yellow to orange and red, enabling semi-qualitative detection of urea via naked eye in a linear range of 0–0.7 M covering a cut-off value of 0.3 M, which allow for distinguishing between the chronic kidney-prone patients from the normal individuals. The hydrogel was found to be non-toxic and demonstrated effective enzyme preservation by maintaining more than 80% of urease activity up to 14 days. This hydrogel-based urea sensor was also validated by laser desorption ionization mass spectrometry (LDI-MS) with satisfactory results. This platform demonstrated its potential integration on diaper for real-time screening of urea in the point-of-care diagnostics of chronic kidney disease (CKD).</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07060-w","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Zwitterionic hydrogel based on a dual-crosslinked network of pluronic F-127 dimethacrylate (PLU-DMA) and a terpolymer, poly(sulfobetaine methacrylate)-co-methacrylic acid-co–N-methacryloyloxyethyl tyrosine methylester) (PSBMM) was prepared and successfully applied as an enzyme-based colorimetric sensor of urea on diaper. The prepared hydrogel possessed good mechanical property while preserving its swelling capability. The urease-incorporated hydrogel exhibited a vivid color change from yellow to orange and red, enabling semi-qualitative detection of urea via naked eye in a linear range of 0–0.7 M covering a cut-off value of 0.3 M, which allow for distinguishing between the chronic kidney-prone patients from the normal individuals. The hydrogel was found to be non-toxic and demonstrated effective enzyme preservation by maintaining more than 80% of urease activity up to 14 days. This hydrogel-based urea sensor was also validated by laser desorption ionization mass spectrometry (LDI-MS) with satisfactory results. This platform demonstrated its potential integration on diaper for real-time screening of urea in the point-of-care diagnostics of chronic kidney disease (CKD).

Graphical abstract

查看原文本刊更多论文

制备了基于pluronic F-127二甲基丙烯酸酯（PLU-DMA）和聚（甲基丙烯酸磺基甜菜碱）-共甲基丙烯酸-共N-甲基丙烯酰氧乙基酪氨酸甲酯（PSBMM）三元共聚物的双交联网络的聚醚型水凝胶，并成功地将其应用于尿布上的酶基尿素比色传感器。制备的水凝胶具有良好的机械性能，同时保持了其溶胀能力。加入尿素酶的水凝胶呈现出从黄色到橙色和红色的鲜艳颜色变化，可在 0-0.7 M 的线性范围内通过肉眼对尿素进行半定量检测，临界值为 0.3 M，可用于区分慢性肾病患者和正常人。研究发现，这种水凝胶无毒，并能有效保存酶，在 14 天内保持 80% 以上的尿素酶活性。这种水凝胶尿素传感器还通过激光解吸电离质谱法（LDI-MS）进行了验证，结果令人满意。该平台证明了其在尿布上的整合潜力，可用于慢性肾病（CKD）护理点诊断中的尿素实时筛查。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.