Characterization of Campylobacter fetus adherence, invasiveness, and ultrastructural damage on bovine oviductal cells.

IF 3.3 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-02-26 DOI:10.1016/j.micpath.2025.107429

Cagnoli Claudia Inés, Chiapparrone María Laura, Acuña Francisco, Cacciato Claudio Santiago, Rodríguez Marcelo Gastón, Aller Juan Florencio, Catena María Del Carmen

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引用次数: 0

Abstract

Bovine campylobacteriosis is a distributed worldwide disease caused by Campylobacter fetus. It is a sexual transmitted disease that affects reproductive health in cattle. The objective of this study was to use primary bovine oviduct cells as a pathogenicity model to study the virulence of Campylobacter fetus fetus and Campylobacter fetus venerealis. Both subspecies showed the ability to adhere to, invade and cause cytopathogenic effect in oviductal cells. Transmission electron microscopy revealed significant ultrastructural damage, including cytoplasmic vacuolization, nuclear condensation and mitochondrial alterations. These mitochondrial changes, such enlargement and fragmentation, suggest potential impacts on cell viability and host cellular metabolism. This study is the first to investigate the pathogenicity of both subspecies in primary bovine oviductal epithelial cells. These findings demonstrate the virulence mechanism of C. fetus in the reproductive tract, providing insights into oviductal pathogenicity and potential impacts on bovine fertility.

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)