Reward expectation and receipt differentially modulate the spiking of accumbens D1+ and D2+ neurons.

Abstract

The nucleus accumbens (NAc) helps govern motivation to pursue reward. Two distinct sets of NAc projection neurons-expressing dopamine D1 vs. D2 receptors-are thought to promote and suppress motivated behaviors, respectively. However, support for this conceptual framework is limited: in particular, the spiking patterns of these distinct cell types during motivated behavior have been largely unknown. Using optogenetic tagging, we recorded the spiking of identified D1+ and D2+ neurons in the NAc core as unrestrained rats performed an operant task in which motivation to initiate work tracks recent reward rate. D1+ neurons preferentially increased firing as rats initiated trials and fired more when reward expectation was higher. By contrast, D2+ cells preferentially increased firing later in the trial, especially in response to reward delivery-a finding not anticipated from current theoretical models. Our results provide new evidence for the specific contribution of NAc D1+ cells to self-initiated approach behavior and will spur updated models of how D2+ cells contribute to learning.