Chitosan-based xerogel film incorporating Nystatin: Synthesis, structural Analysis, and biological evaluation

IF 4.2 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 2025-02-26 DOI:10.1016/j.ymeth.2025.02.011

Zahra Zareshahrabadi , Sara Shenavari , Forough Karami , Mohammad Hashem Hashempur , Mohammad Khorram , Ali Arabimonfard , Mahboobeh Jafari , Ali Mohammad Tamaddon , Gholamhossein Yousefi , Kamiar Zomorodian

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引用次数: 0

Abstract

Wound infections are challenging to manage, requiring innovative wound dressing systems with prescribed properties. Active wound dressings should provide a moist environment, protect from secondary infections, and remove wound exudate to accelerate tissue regeneration. Hydrogels are encouraging matrices for bioactive compound encapsulation in pharmaceutical applications. The aim of the present study is to design chitosan/gelatin /polyvinyl alcohol-based xerogel film containing nystatin for wound dressing applications with antifungal properties. The xerogel film is developed using a film-casting method and evaluated for its chemical and physical characteristics using FTIR, SEM, AFM, and tensile analysis. Water barrier properties of the film, such as the moisture content (17.84 ± 3.62 %), water solubility (44.50 ± 5.55 %), gel fraction (55.50 ± 5.55 %), and water vapour transmission rate (1912.25 ± 248.12 g m⁻² per day), suggest a humid microenvironment suitable for wound. The xerogel film, characterized by its robust mechanical strength, substantial swelling capacity (∼120–400 %) across various pH levels, and acceptable bio-adhesive properties, reveals as a potential antifungal wound dressing material. In vitro toxicity assessments confirm its biocompatibility towards both RBCs and NIH-3 T3 fibroblast cells. The findings confirm that the film formulation has strong antifungal properties, with a minimum inhibitory concentration of 2–8 μL/mL against Candida species, as well as outstanding antibiofilm effectiveness (∼85 %) and a significant reduction of the fungal colony count (∼100 %). Moreover, its controlled drug release capabilities, along with antifungal properties, offer it as an appealing dressing for the localized treatment of superficial fungal infections. As a result, this xerogel film can be used as a versatile platform for advanced wound care and therapeutic applications.

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来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.