Hematological Profile of Naturally Infected Long-Tailed Macaques (Macaca fascicularis) With Plasmodium Inui in Ex Situ Captivity Prior to Ex Vivo Cultivation

IF 0.8 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Medical Primatology Pub Date : 2025-03-02 DOI:10.1111/jmp.70011

Risqa Novita, Agik Suprayogi, Andria Agusta, Arifin Budiman Nugraha, Huda Shalahudin Darusman

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引用次数: 0

Abstract

Background

Anemia, characterized by low hemoglobin, is a key malaria indicator. Malaria in non-human primates (NHPs) is often asymptomatic, requiring laboratory tests and microscopy for diagnosis. Hematological profiles are crucial for understanding NHP clinical conditions and supporting ex vivo Plasmodium culture for antimalarial testing. This study examines hematological changes in Macaca fascicularis naturally infected with Plasmodium inui and provides hematocrit guidelines for ex vivo culture.

Methods

Ten M. fascicularis were diagnosed with Plasmodium inui in 2023 and subsequently re-screened in this study through microscopic and molecular analyses. Two monkeys (M4, M10) with the highest parasitemia and one negative control (M6) were analyzed. Hematological, albumin, and glucose levels were measured.

Results

M4 and M10 showed 34.5% and 30.2% hematocrit values, with hemoglobin levels of 10.5 and 8.6 g/dL. M10 exhibited anemia and thrombocytopenia.

Conclusions

M10 showed hematological signs of malaria, while all macaques exhibited microcytic hypochromic anemia. High hematocrit levels are recommended for ex vivo culture.

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来源期刊

Journal of Medical Primatology 生物-动物学

CiteScore

1.40

自引率

42.90%

发文量

审稿时长

6 months

期刊介绍： The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs. Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.