Effects of valproic acid, levetiracetam, carbamazepine, lamotrigine, and topiramate on LIF, E-cadherin, and FOXO1 mediator molecules in rat embryo implantation

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology Pub Date : 2025-02-26 DOI:10.1016/j.fct.2025.115352

Pınar Özkan Kart , Nihal Yıldız , Seren Gülşen Gürgen , Hayrunnisa Yeşil Sarsmaz , Ali Cansu

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引用次数: 0

Abstract

Background

This study investigated the effects of valproic acid (VPA), levetiracetam (LEV), carbamazepine (CBZ), lamotrigine (LTG), and topiramate (TPM) on LIF, E-cadherin, and FOXO1 mediator molecules during implantation in rat embryos.

Materials and methods

Sixty female rats were divided into six experimental groups, and the control solution and drugs were administered by gavage for 90 days. At the end of three months, implantation sites were obtained, and histological and immunohistochemical staining protocols were applied.

Results

Embryonic trophectoderm cells were surrounded by inflammatory cells in the VPA group. Increased eosinophilic staining was seen in the primary decidual zone cells in the CBZ group, mast cells in the LTG group, and intense inflammatory cells in the TMP group. LIF staining in the VPA, CBZ, LTG, and TPM groups showed weak to moderate LIF expression (p < 0.001). In E-cadherin staining, the LTG group showed moderate and the TPM group showed weak immune reactions (p < 0.001). Embryonic cells and primary decidual zone cells in control, LEV, CBZ, and LTG groups showed weak to strong expression of FOXO1, while VPA and TPM groups showed no reaction (p < 0.001).

Conclusions

In summary, antiseizure medication use had a negative effect on the expression of proteins that play key roles in embryo implantation in young non-epileptic rats to varying degrees.

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.