Recent developments of imaged capillary isoelectric focusing technology for in-depth biopharmaceutical characterization

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-02-26 DOI:10.1016/j.trac.2025.118212

Tao Bo , Feng Yang , Bo Yan , David A. Michels , Tiemin Huang , Janusz Pawliszyn

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引用次数: 0

Abstract

Imaged capillary isoelectric focusing (icIEF) has made remarkable strides in recent years, presenting the biopharmaceutical industry with a range of innovative solutions to characterize protein charge heterogeneity and streamline the development and manufacturing of biologics. This review highlights the latest advancements in icIEF technology for comprehensive biopharmaceutical characterization. Key innovations, including the development of critical reagents and capillary coatings, advanced icIEF fractionation, cutting-edge icIEF-MS online coupling, and novel applications for protein bio-interactions, are redefining icIEF analytical methodologies and broadening their applicability. These breakthroughs significantly enhance the characterization of complex therapeutic proteins, aiding researchers in mitigating challenges and setbacks during drug development and manufacturing. In describing these advances, we delve into multidisciplinary concepts spanning biology, chemistry, instrument design, and workflow optimization, aiming to inspire further innovations and insights in this dynamic, rapidly evolving field. Furthermore, this review traces the origins and 30-year evolution of icIEF technology by illustrating its continuous progression and expanding impact over time.

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.