Caution, these are glycan sulfates. The features of their interaction with proteins

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-02-20 DOI:10.1016/j.carres.2025.109433

Nicolai Bovin , Marina Sablina , Galina Pazynina , Polina Obukhova , Aligeydar Ragimov , Nadezhda Shilova

引用次数: 0

Abstract

In studies of viruses, lectins and especially human blood anti-glycan antibodies using printed glycan array (PGA), sulfated glycans suspiciously often turn out to be the highest-level binders. The binding to sulfated glycan along with parent neutral is easily explained by the similarity of these two glycans, while the unexpected thing is the many times stronger binding. Analysis of data accumulated over almost two decades allows us to explain the observed effect by the Coulomb interaction of the sulfate residue with a positively charged amino acid that accidently appears near the binding site of the neutral glycan backbone. That is, there is an effect of enhancing the specific interaction by an additional electrostatic one. It is expected that the material considered in the article will be useful for the correct interpretation of other data on the specificity of proteins capable of binding charged glycans, which are often encountered in nature.

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注意，这些是糖硫酸盐。它们与蛋白质相互作用的特点

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".