Method validation and quantification of 188 pesticides in animal liver using QuEChERS and GC-MS/MS

IF 1.8 Q4 TOXICOLOGY
Lavanya K. Vijaykumar, Dharanesha N. Krishnegowda, Sneha Judith, Lohith T. Shankarappa, Asha Mayanna, Jagadeesh S. Sanganal, Raveendra Hegde
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引用次数: 0

Abstract

The pesticide residues in animal products are the main research focus in the present scenario since they pose a severe health hazard to humans and also loss for the livestock industry. The goal of the current work was to create a method and screen animal liver from Karnataka, India for pesticide residues (n = 188) using gas chromatography triple quadrupole mass spectrometry. We found strong linearity (r2 = 0.999) from 50 to 800 ppb for the pesticides standard mixture. The lowest limit of detection (LOD) of 0.067 ppb was found for pebulate compound, followed for endosulfan ether was 0.07 ppb, and we found highest LOD for dichlofluanid (0.986 ppb). Coming to the limit of quantification, lowest was found for pebulate (0.202 ppb), followed by endosulfan ether (0.212 ppb), and the highest was obtained in dichlofluanid (2.989 ppb). The average recovery ranged from 84 to 122% and 81 to 105%, at 100 and 400 ppb, respectively. In the initial study, we analyzed 31 liver samples that were sent to our Veterinary Forensic Science Laboratory between February 2022 and October 2023 and pesticide residues have been quantified in 9 samples from domestic as well as wild animals. A total of eighteen pesticides from the organonitrogen compounds (n = 8), organochlorine (n = 7), organophosphorus (n = 2), and pyrethroids (n = 1) categories were found and none were quantified higher than the maximum residue limits set by different national and international regulatory bodies except for beta benzene hexachloride and chlorpyrifos. It can be concluded that our developed method using a QuEChERS extraction or cleanup method and triple quadrupole mass spectrometry can be applied to the regular monitoring of the majority of multi-residue pesticides in less time and thereby control management can be initiated.
方法采用QuEChERS和GC-MS/MS对动物肝脏中188种农药进行定量验证
动物产品中的农药残留是目前情况下的主要研究重点,因为它们对人类健康造成严重危害,也给畜牧业造成损失。当前工作的目标是创建一种方法,并使用气相色谱三重四极杆质谱法筛选来自印度卡纳塔克邦的动物肝脏中农药残留(n = 188)。在50 ~ 800 ppb范围内线性关系良好(r2 = 0.999)。其中,珠酸盐化合物的最低检出限为0.067 ppb,硫丹醚的最低检出限为0.07 ppb,二氯氟醚的最高检出限为0.986 ppb。在达到定量极限时,最小的是珍珠酸盐(0.202 ppb),其次是硫丹醚(0.212 ppb),最高的是二氯氟醚(2.989 ppb)。在100 ppb和400 ppb下,平均采收率分别为84 ~ 122%和81 ~ 105%。在初步研究中,我们分析了2022年2月至2023年10月间送到兽医法医学实验室的31份肝脏样本,并对来自家养和野生动物的9份样本中的农药残留进行了量化。从有机氮化合物(n = 8)、有机氯(n = 7)、有机磷(n = 2)和拟除虫菊酯(n = 1)类农药中共检出18种农药,除六氯化苯和毒死蜱外,均未超过不同国家和国际监管机构规定的最大残留限量。结果表明,采用QuEChERS萃取或清除法和三重四极杆质谱法的方法可在较短的时间内对大多数多残留农药进行定期监测,从而启动控制管理。
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来源期刊
CiteScore
0.90
自引率
33.30%
发文量
393
审稿时长
47 days
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