Effects of digestate soil amendment on the fate of trace metals and on the soil microbial community

IF 7.6 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Environmental Pollution Pub Date : 2025-03-01 DOI:10.1016/j.envpol.2025.125961

Veronica Baldasso, Maria Paola Tomasino, Stéphanie Sayen, Emmanuel Guillon, Luigi Frunzo, Carlos A.R. Gomes, Maria João Alves, Ricardo Castro, Ana Paula Mucha, C. Marisa R. Almeida

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Abstract

This study evaluated, over a 28-day soil column experiment, (i) the fate of trace metals (Zn, Cu, Pb) in a loamy sand soil after amendment with a non-source-separated municipal solid waste (OFMSW) digestate; (ii) the impact of the OFMSW digestate on soil microbial community; and (iii) the effects of two pharmaceuticals (metformin and lamotrigine) on trace metal fate and microbial community dynamics. Three conditions were tested: natural digestate, digestate spiked with metformin, and digestate spiked with lamotrigine. Soil samples were collected over time to measure trace metal concentrations and fractionation and characterize the soil prokaryotic community using Illumina next generation sequencing technology.Results showed Pb mobility factor (MF) increased over time by 182% in amended soil and 126% in the other soil layers, while Zn MF increased by 85% and decreased by 36%, respectively. Total metal concentrations were, nevertheless, low, pointing out that the impact of these metals on the soil in this study should be low. Cu MF remained constant (ca. 1.75%). Digestate amendment increased soil microbial diversity, with Shannon Index rising from 4.9 to 5.6, and shifted its composition over time, promoting a more diverse community. Initially dominated by Firmicutes, it stabilised by day 14 with Proteobacteria and Bacteroidota becoming dominant. Pharmaceuticals at environmentally relevant concentrations did not significantly affect metal behaviour or prokaryotic community structure.These findings suggest digestate may immobilize contaminants, making it a promising resource for soil improvement practices. However, preliminary treatment and monitoring are crucial for its safe application within circular bioeconomy strategies.

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本研究在为期 28 天的土壤柱实验中评估了：(i) 使用未分离来源的城市固体废物（OFMSW）沼渣进行改良后，壤质砂土中痕量金属（锌、铜、铅）的归宿；(ii) OFMSW 沼渣对土壤微生物群落的影响；以及 (iii) 两种药物（二甲双胍和拉莫三嗪）对痕量金属归宿和微生物群落动态的影响。测试了三种情况：天然沼渣、添加二甲双胍的沼渣和添加拉莫三嗪的沼渣。结果表明，随着时间的推移，铅迁移系数（MF）在改良土壤中增加了 182%，在其他土层中增加了 126%，而锌迁移系数分别增加了 85%和减少了 36%。不过，总金属浓度较低，说明这些金属对本研究中的土壤影响较小。铜 MF 保持不变（约 1.75%）。沼渣添加剂提高了土壤微生物的多样性，香农指数从 4.9 上升到 5.6，并且随着时间的推移改变了其组成，促进了群落的多样性。最初以固着菌为主，到第 14 天时趋于稳定，变形菌和类杆菌开始占主导地位。这些研究结果表明，沼渣可以固定污染物，使其成为土壤改良实践中一种很有前景的资源。不过，要在循环生物经济战略中安全应用沼渣，初步处理和监测至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental Pollution 环境科学-环境科学

CiteScore

16.00

自引率

6.70%

发文量

2082

审稿时长

2.9 months

期刊介绍： Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health. Subject areas include, but are not limited to: • Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies; • Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change; • Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects; • Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects; • Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest; • New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.