[Serological and Molecular Biological Characteristics of cisAB Blood Group and Transfusion Strategies].

Q4 Medicine

中国实验血液学杂志 Pub Date : 2025-02-01 DOI:10.19746/j.cnki.issn.1009-2137.2025.01.030

Si-Meng Wu, Qiao-Ni Yang, Wa Gao, Xiao-Shuai Li, Qiu-Shi Wang

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引用次数: 0

Abstract

Objective: To analyze the serological and molecular biological characteristics of 5 patients with cis AB blood group, and to explore the safe transfusion strategy.

Methods: Serological identification of the samples' blood group was performed using anti-A, anti-B, anti-D, anti-A1, anti-H typing reagents and ABO reagent erythrocytes. Molecular biological identification of the samples' blood group was performed using PCR-SSP or gene sequencing.

Results: The serological identification results of blood group in 5 patients all showed inconsistent forward and reverse typing, presenting as A₂B₃ or A₂B_w. ABO gene sequencing of samples 1, 2 and 3 showed 261delG in exon 6 and 467C>T, 803G>C in exon 7. The genotypes of samples 1, 2 and 3 were determined to be cisAB/O . PCR-SSP genotyping was performed on sample 4 and 5，and the results were both cisAB/O .

Conclusion: Patients with cisAB alleles have inconsistent serological manifestations, and genetic testing is necessary to ensure the safety and effectiveness of blood transfusion.

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[cisAB血型的血清学和分子生物学特性及输血策略]。

目的：分析5例顺式AB血型患者的血清学及分子生物学特征，探讨安全输血策略。方法：采用抗a、抗b、抗d、抗a1、抗h分型试剂及ABO试剂进行血清学鉴定。采用PCR-SSP或基因测序对样本血型进行分子生物学鉴定。结果：5例患者血清学血型鉴定结果均为正反分型不一致，表现为A2B3或A2Bw。样品1、2、3的ABO基因测序结果显示，第6外显子为261delG，第7外显子为467C>T, 803G>C。样品1、2、3的基因型均为cisAB/O型。对样本4和样本5进行PCR-SSP基因分型，结果均为cisAB/O。结论：携带cisAB等位基因的患者血清学表现不一致，为确保输血安全有效，进行基因检测是必要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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