[Screening and Preliminary Validation of Multiple Myeloma Specific Proteins].

Q4 Medicine

中国实验血液学杂志 Pub Date : 2025-02-01 DOI:10.19746/j.cnki.issn.1009-2137.2025.01.018

Shan Zhao, Hui-Hui Liu, Xiao-Ying Yang, Wei-Wei Xie, Chao Xue, Xiao-Ya He, Jin Wang, Yu-Jun Dong

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引用次数: 0

Abstract

Objective: To screen novel diagnostic marker or therapeutic target for multiple myeloma (MM).

Methods: Sel1L, SPAG4, KCNN3 and PARM1 were identified by bioinformatics method based on GEO database as high expression genes in MM. Their RNA and protein expression levels in bone marrow mononuclear cells from myeloma cell lines U266, NCI-H929, MM.1s, RPMI8226 and leukemia cell line THP1, as well as 31 MM patients were evaluated by RT-PCR and Western blot, respectively. Meanwhile, 5 samples of bone marrow from healthy donors for allogeneic hematopoietic stem cell transplantation were employed as controls.

Results: Compared with leukemia cell line THP1, the expression levels of KCNN3, PARM1 and Sel1L mRNA were significantly increased in myeloma cell lines U266, NCI-H929 and MM.1s, while PARM1 was further increased in myeloma cell lines 8226. Western blot showed that the 4 genes were all expressed in the 4 myeloma cell lines. Compared with healthy controls, the expression levels of Sel1L, SPAG4, KCNN3 and PARM1 mRNA were significantly higher in MM patients (all P < 0.05). Western blot showed that the 4 genes were all expressed in MM patients, and the protein expression level of Sel1L and KCNN3 were significantly different compared with healthy donors (all P < 0.01).

Conclusion: Sel1L, SPAG4, KCNN3 and PARM1 may be potential diagnostic markers and therapeutic targets for MM.

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[多发性骨髓瘤特异性蛋白的筛选和初步验证]。

目的：筛选新的多发性骨髓瘤（MM）诊断标志物或治疗靶点。方法：采用基于GEO数据库的生物信息学方法，鉴定出Sel1L、SPAG4、KCNN3和PARM1为MM高表达基因，分别采用RT-PCR和Western blot方法检测其在骨髓瘤细胞系U266、NCI-H929、MM.1s、RPMI8226、白血病细胞系THP1和31例MM患者骨髓单个核细胞中的RNA和蛋白表达水平。同时选取5例健康供者骨髓作为对照，用于同种异体造血干细胞移植。结果：与白血病细胞系THP1相比，骨髓瘤细胞系U266、NCI-H929和MM.1s中KCNN3、PARM1和Sel1L mRNA的表达水平显著升高，而骨髓瘤细胞系8226中PARM1的表达水平进一步升高。Western blot结果显示，这4个基因在4株骨髓瘤细胞株中均有表达。MM患者Sel1L、SPAG4、KCNN3、PARM1 mRNA的表达水平较健康对照组显著升高（均P < 0.05）。Western blot结果显示，4个基因在MM患者中均有表达，且Sel1L、KCNN3蛋白表达量与健康供者相比差异有统计学意义（P < 0.01）。结论：Sel1L、SPAG4、KCNN3和PARM1可能是MM的潜在诊断标志物和治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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