Potable water as a source of intermediate and borderline-resistant Aspergillus and Candida strains.

Abstract

The World Health Organization calls to assess possible health risks from emerging fungi originating not only from hospitals but also from the natural environment. Fungal contamination in oligotrophic water systems represents a public health concern due to the potential for the emergence of antifungal-resistant strains. This study focused on the identification of Aspergillus spp. and Candida spp. isolated from different water sources and materials in contact with water. Isolated strains have been tested against nine antifungals to assess the prevalence of resistance in these strains. Only one strain of Aspergillus protuberus was resistant to amphotericin B. On the other hand, all Candida strains were intermediately resistant to anidulafungin and micafungin, 5.8% were borderline resistant to 5-flucytosine and fluconazole, and 3% to voriconazole. Candida parapsilosis sensu stricto isolated from water samples had statistically higher minimal inhibitory concentration (MIC) for anidulafungin than clinical strains and clinical strains had statistically higher MIC for itraconazole. Statistical analysis pointed out habitat to be significant for higher MIC in C. parapsilosis. Our findings show that borderline-resistant strains can be transferred by water; thus, potable water should be considered as a possible source of resistant strains in hospitals and healthcare units.