Predominance of Auditory but not Vestibular Deficits in the Mouse Model of Congenital Cytomegalovirus Infection.

IF 2.9 3区医学 Q1 OTORHINOLARYNGOLOGY

Clinical and Experimental Otorhinolaryngology Pub Date : 2025-02-28 DOI:10.21053/ceo.2024.00305

Chi-Chieh Chang, Ying-Chang Lu, Po-Nien Tsao, Yen-Hui Chan, Yi-Shan Lee, Pei-Hsuan Lin, Cheng-Yu Hsieh, Wei-Chung Hsu, Chuan-Jen Hsu, Chun-Yi Lu, Chen-Chi Wu

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引用次数: 0

Abstract

Objective: The aim of this study was to investigate the auditory and vestibular consequences of congenital cytomegalovirus (cCMV) infection in a C57BL/6 mouse model established by intraperitoneal inoculation and designed to mimic clinical phenotypes.

Methods: A cCMV infection model was established by intraperitoneal inoculation of murine cytomegalovirus into C57BL/6 mice. Auditory and vestibular function were assessed by auditory brainstem response, open field, swim, and rotarod tests. Histologic evaluation of the inner ear was also performed.

Results: The study successfully established a model of cCMV infection in C57BL/6 mice. Auditory deficits of varying severity and laterality were observed, reflecting the clinical spectrum in humans. Vestibular function was minimally affected and did not correlate with auditory deficits. Histopathologic examination revealed predominant damage to spiral ganglion neurons with relatively intact vestibular organs.

Conclusion: Our optimized mouse model effectively recapitulates the inner ear manifestations of human cCMV infection. The predominance of auditory over vestibular deficits provides valuable insights for the development of targeted therapeutic interventions for inner ear sequelae of cCMV infection.

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先天性巨细胞病毒感染小鼠模型中听觉而非前庭功能缺损的优势。

目的：本研究旨在探讨先天性巨细胞病毒（cCMV）感染C57BL/6小鼠模型的听觉和前庭功能的影响，该模型通过腹腔接种模拟临床表型。方法：采用小鼠巨细胞病毒腹腔接种C57BL/6小鼠，建立cCMV感染模型。听觉和前庭功能通过听觉脑干反应、开放场、游泳和旋转测试来评估。对内耳进行组织学检查。结果：本研究成功建立了C57BL/6小鼠cCMV感染模型。观察到不同程度和侧边的听觉缺陷，反映了人类的临床频谱。前庭功能受影响最小，与听觉缺陷无关。组织病理学检查显示螺旋神经节神经元损伤为主，前庭器官相对完整。结论：我们优化的小鼠模型有效地再现了人cCMV感染的内耳表现。听觉缺陷在前庭缺陷中的优势为cCMV感染内耳后遗症的靶向治疗干预的发展提供了有价值的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Otorhinolaryngology 医学-耳鼻喉科学

CiteScore

4.90

自引率

6.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Clinical and Experimental Otorhinolaryngology (Clin Exp Otorhinolaryngol, CEO) is an international peer-reviewed journal on recent developments in diagnosis and treatment of otorhinolaryngology-head and neck surgery and dedicated to the advancement of patient care in ear, nose, throat, head, and neck disorders. This journal publishes original articles relating to both clinical and basic researches, reviews, and clinical trials, encompassing the whole topics of otorhinolaryngology-head and neck surgery. CEO was first issued in 2008 and this journal is published in English four times (the last day of February, May, August, and November) per year by the Korean Society of Otorhinolaryngology-Head and Neck Surgery. The Journal aims at publishing evidence-based, scientifically written articles from different disciplines of otorhinolaryngology field. The readership contains clinical/basic research into current practice in otorhinolaryngology, audiology, speech pathology, head and neck oncology, plastic and reconstructive surgery. The readers are otolaryngologists, head and neck surgeons and oncologists, audiologists, and speech pathologists.