{"title":"In vitro use of a low-level laser therapy device inhibits growth of Malassezia pachydermatis.","authors":"Natalia Gil, Domenico Santoro, Rosanna Marsella","doi":"10.2460/ajvr.24.11.0355","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To determine the in vitro activity of the low-level laser (light) therapy (LLLT) device (Phovia; Vetoquinol) on Malassezia pachydermatis.</p><p><strong>Methods: </strong>Clinical isolates of M pachydermatis (n = 30) and a commercially available anamorph of M pachydermatis (ATCC strain 14522) were used in this study. Both groups of organisms were plated on potato agar plates and exposed to the LLLT device for 2, 4, 6, and 8 minutes with a 30-second break after every 2 minutes of exposure. The plates were then incubated at 37 °C for 48 hours. Each experiment was run in duplicate. The experiment for the internal control was repeated independently 6 times. Nonirradiated plates served as the viability control (positive control). The number of CFUs between each treatment and the control was analyzed using a repeated-measures ANOVA or a Friedman test followed by a post hoc analysis.</p><p><strong>Results: </strong>Compared to the control, there was a statistically significant decline in CFUs after a minimum of 4 minutes of exposure to the LLLT device in both groups compared to unexposed controls.</p><p><strong>Conclusions: </strong>This preliminary in vitro study demonstrated that the LLLT device tested can inhibit the growth of M pachydermatis.</p><p><strong>Clinical relevance: </strong>This in vitro study offers a novel proof-of-concept approach to treating Malassezia infections in veterinary medicine. Low-level laser (light) therapy has the potential to shorten treatment durations and reduce side effects, making it a promising alternative to standard antifungal therapy, particularly in the context of antifungal resistance.</p>","PeriodicalId":7754,"journal":{"name":"American journal of veterinary research","volume":" ","pages":"1-6"},"PeriodicalIF":1.3000,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of veterinary research","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2460/ajvr.24.11.0355","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To determine the in vitro activity of the low-level laser (light) therapy (LLLT) device (Phovia; Vetoquinol) on Malassezia pachydermatis.
Methods: Clinical isolates of M pachydermatis (n = 30) and a commercially available anamorph of M pachydermatis (ATCC strain 14522) were used in this study. Both groups of organisms were plated on potato agar plates and exposed to the LLLT device for 2, 4, 6, and 8 minutes with a 30-second break after every 2 minutes of exposure. The plates were then incubated at 37 °C for 48 hours. Each experiment was run in duplicate. The experiment for the internal control was repeated independently 6 times. Nonirradiated plates served as the viability control (positive control). The number of CFUs between each treatment and the control was analyzed using a repeated-measures ANOVA or a Friedman test followed by a post hoc analysis.
Results: Compared to the control, there was a statistically significant decline in CFUs after a minimum of 4 minutes of exposure to the LLLT device in both groups compared to unexposed controls.
Conclusions: This preliminary in vitro study demonstrated that the LLLT device tested can inhibit the growth of M pachydermatis.
Clinical relevance: This in vitro study offers a novel proof-of-concept approach to treating Malassezia infections in veterinary medicine. Low-level laser (light) therapy has the potential to shorten treatment durations and reduce side effects, making it a promising alternative to standard antifungal therapy, particularly in the context of antifungal resistance.
期刊介绍：
The American Journal of Veterinary Research supports the collaborative exchange of information between researchers and clinicians by publishing novel research findings that bridge the gulf between basic research and clinical practice or that help to translate laboratory research and preclinical studies to the development of clinical trials and clinical practice. The journal welcomes submission of high-quality original studies and review articles in a wide range of scientific fields, including anatomy, anesthesiology, animal welfare, behavior, epidemiology, genetics, heredity, infectious disease, molecular biology, oncology, pharmacology, pathogenic mechanisms, physiology, surgery, theriogenology, toxicology, and vaccinology. Species of interest include production animals, companion animals, equids, exotic animals, birds, reptiles, and wild and marine animals. Reports of laboratory animal studies and studies involving the use of animals as experimental models of human diseases are considered only when the study results are of demonstrable benefit to the species used in the research or to another species of veterinary interest. Other fields of interest or animals species are not necessarily excluded from consideration, but such reports must focus on novel research findings. Submitted papers must make an original and substantial contribution to the veterinary medicine knowledge base; preliminary studies are not appropriate.
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