Effect of basic-fibroblast growth factor on tympanic membrane perforation in a mouse model of blast injury.

IF 1.2 4区 医学 Q3 OTORHINOLARYNGOLOGY
Motohiro Sato, Kunio Mizutari, Takaomi Kurioka, Yoshiaki Inuzuka, Shingo Yasutake, Koji Araki, Yasushi Satoh, Akihiro Shiotani
{"title":"Effect of basic-fibroblast growth factor on tympanic membrane perforation in a mouse model of blast injury.","authors":"Motohiro Sato, Kunio Mizutari, Takaomi Kurioka, Yoshiaki Inuzuka, Shingo Yasutake, Koji Araki, Yasushi Satoh, Akihiro Shiotani","doi":"10.1080/00016489.2025.2470971","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Tympanic membrane perforation (TMP) caused by blast injury result in poor TMP closure and pathological regeneration of the tympanic membrane (TM). TM regeneration therapy using basic fibroblast growth factor (bFGF) has demonstrated good outcomes for chronic otitis media.</p><p><strong>Aims/objectives: </strong>To investigate the efficacy of bFGF treatment in the management of blast-induced TMP.</p><p><strong>Material and methods: </strong>Male CBA/J mice (10-12-week-old) were divided into the bFGF-treatment and no-treatment groups. Blast-tube systems were used to induce TMP. Auditory evaluation, TM observation, histological evaluation of the regenerated TM, and histological evaluation of the inner ear were conducted.</p><p><strong>Results: </strong>Auditory assessment revealed no difference between the groups, with persistent auditory threshold elevation being observed even 1 month post-injury. Similarly, histological evaluation of the inner ear hair cells revealed no significant differences between the groups; no hair cell death was evident. However, the regenerated TM in the bFGF-treated group was significantly thicker than that in the untreated group.</p><p><strong>Conclusions and significance: </strong>A good TMP closure rate was observed in the present study; however, bFGF treatment exacerbated thickening of the regenerated TM. Future studies must study the therapeutic effects of bFGF treatment using various animal models and timing and concentration of bFGF treatment.</p>","PeriodicalId":6880,"journal":{"name":"Acta Oto-Laryngologica","volume":" ","pages":"1-8"},"PeriodicalIF":1.2000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Oto-Laryngologica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/00016489.2025.2470971","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OTORHINOLARYNGOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Tympanic membrane perforation (TMP) caused by blast injury result in poor TMP closure and pathological regeneration of the tympanic membrane (TM). TM regeneration therapy using basic fibroblast growth factor (bFGF) has demonstrated good outcomes for chronic otitis media.

Aims/objectives: To investigate the efficacy of bFGF treatment in the management of blast-induced TMP.

Material and methods: Male CBA/J mice (10-12-week-old) were divided into the bFGF-treatment and no-treatment groups. Blast-tube systems were used to induce TMP. Auditory evaluation, TM observation, histological evaluation of the regenerated TM, and histological evaluation of the inner ear were conducted.

Results: Auditory assessment revealed no difference between the groups, with persistent auditory threshold elevation being observed even 1 month post-injury. Similarly, histological evaluation of the inner ear hair cells revealed no significant differences between the groups; no hair cell death was evident. However, the regenerated TM in the bFGF-treated group was significantly thicker than that in the untreated group.

Conclusions and significance: A good TMP closure rate was observed in the present study; however, bFGF treatment exacerbated thickening of the regenerated TM. Future studies must study the therapeutic effects of bFGF treatment using various animal models and timing and concentration of bFGF treatment.

碱性成纤维细胞生长因子对爆炸伤小鼠模型鼓膜穿孔的影响
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Acta Oto-Laryngologica
Acta Oto-Laryngologica 医学-耳鼻喉科学
CiteScore
2.50
自引率
0.00%
发文量
99
审稿时长
3-6 weeks
期刊介绍: Acta Oto-Laryngologica is a truly international journal for translational otolaryngology and head- and neck surgery. The journal presents cutting-edge papers on clinical practice, clinical research and basic sciences. Acta also bridges the gap between clinical and basic research.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信