Amirhossein Favakeh, Amir Mokhtare, Mohammad Javad Asadi, James C M Hwang, Alireza Abbaspourrad
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引用次数: 0
Abstract
The use of the intrinsic electrical properties of a single cell by broadband electrical impedance spectroscopy (EIS) as a label-free and non-invasive method to monitor cellular and intracellular features is an emerging field. Here, we present a novel EIS-based sheathless microfluidic platform with an integrated coplanar waveguide to probe the interior of a single cell. This platform allows for precise single-cell trapping by dielectrophoresis, hydrodynamic focusing, and sensing the electrical properties of the trapped single cell. We measured the impedance characteristics of a single Schizosaccharomyces pombe (fission) yeast cell by a single frequency sweep (30 kHz to 6GHz) in a stagnant sucrose solution using two-port scattering (S) parameters. The measurements revealed a clear distinction between the cytoplasm impedance of live versus dead cells at 3 GHz. This platform could provide real-time monitoring of cellular electrical responses to chemical and physical antagonists for diagnostic purposes.
期刊介绍：
Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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