Comparative Study of Fluorophores for Precise Dopamine Detection and Investigation of Its Association with Stress and Coffee Addiction in HEK 293 Cells.
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Abstract
In today's world, where stress and addiction are increasingly prevalent due to job pressures and coping mechanisms, dopamine (Dopa), a key hormone linked to mood, happiness, and mental health, has become vital for understanding conditions like depression and anxiety. Our study focuses on detecting Dopa pathways both in vitro using HEK293 cells and in vivo using zebrafish under stress and addiction conditions. We employed a biocompatible organic fluorophore (P1), with pyrazole-4-carboxaldehyde as the recognition unit, which demonstrated a detection limit of 8.2 nM, aligning with physiological Dopa levels. P1's efficacy in detecting Dopa was validated in human samples (urine, blood, and serum) and artificial samples, confirming its potential for real-world applications. This research is crucial for developing better diagnostic tools and therapies for dopamine-related disorders, offering significant societal benefits for addressing mental health challenges.
期刊介绍：
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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