Genome-wide screen of Escherichia coli Keio mutant line identifies genes related to propolis effect

IF 3 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

European Food Research and Technology Pub Date : 2024-12-18 DOI:10.1007/s00217-024-04642-5

Esra Dibek, Anara Babayeva, Emine Sonay Elgin, Merve Sezer Kürkçü, Bekir Çöl

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Abstract

Propolis, a natural bee product and functional food, exhibits potent antimicrobial properties. This study investigates the effect of propolis on Escherichia coli K-12 using the Keio genome-wide knockout mutant line, which contains single deletions of 3985 non-essential genes. 54 mutants showed sensitivity to propolis at sub-lethal concentrations for the wild type strain. The most sensitive mutants lacked the genes related to iron uptake by ferric enterobactin (FepD, FepA), glutamine transport (GlnP) and peptidoglycan recycling (LdcA). Complementation of these mutants with the corresponding genes restored resistance, suggesting that FepD, FepA, GlnP and LdcA play critical roles in providing intrinsic resistance to the antimicrobial effect of propolis. All 54 sensitive mutants were also classified into highly sensitive (HS, n = 4), moderately sensitive (MS, n = 14) and low sensitive (LS, n = 36) based on the level of growth inhibition. EcoCyc and Omics Dashboard analyses showed that the systems most affected by propolis were regulation, cell exterior, central dogma and other pathways. Enrichment of propolis sensitive gene hits using DAVID pathway analysis revealed the importance of ferric-enterobactin transport and metalloaminopeptidase activities. As a result of testing 3985 mutants, this study sheds light on the antimicrobial effect of propolis and the cellular adaptation of E. coli by providing molecular targets and pathways.

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大肠杆菌Keio突变系全基因组筛选鉴定蜂胶效应相关基因

蜂胶是一种天然的蜂产品和功能性食品，具有很强的抗菌性能。本研究利用Keio全基因组敲除突变系研究蜂胶对大肠杆菌K-12的影响，该突变系包含3985个非必需基因的单缺失。54个突变体对野生型菌株亚致死浓度的蜂胶敏感。最敏感的突变体缺乏铁肠杆菌（FepD, FepA），谷氨酰胺运输（GlnP）和肽聚糖回收（LdcA）的铁摄取相关基因。这些突变体与相应基因的互补恢复了抗性，这表明FepD、FepA、GlnP和LdcA在蜂胶抗菌作用的内在抗性中发挥了关键作用。根据生长抑制程度将54个敏感突变体分为高敏感（HS, n = 4）、中敏感（MS, n = 14）和低敏感（LS, n = 36）。EcoCyc和Omics Dashboard分析表明，受蜂胶影响最大的系统是调控、细胞外部、中枢通路等。利用DAVID通路分析富集蜂胶敏感基因，揭示了铁-肠杆菌蛋白转运和金属氨基肽酶活性的重要性。通过对3985个突变体的测试，本研究通过提供分子靶点和途径，揭示了蜂胶的抗菌作用和大肠杆菌的细胞适应性。

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来源期刊

European Food Research and Technology 工程技术-食品科技

CiteScore

6.60

自引率

3.00%

发文量

232

审稿时长

2.0 months

期刊介绍： The journal European Food Research and Technology publishes state-of-the-art research papers and review articles on fundamental and applied food research. The journal''s mission is the fast publication of high quality papers on front-line research, newest techniques and on developing trends in the following sections: -chemistry and biochemistry- technology and molecular biotechnology- nutritional chemistry and toxicology- analytical and sensory methodologies- food physics. Out of the scope of the journal are: - contributions which are not of international interest or do not have a substantial impact on food sciences, - submissions which comprise merely data collections, based on the use of routine analytical or bacteriological methods, - contributions reporting biological or functional effects without profound chemical and/or physical structure characterization of the compound(s) under research.