The complex of arachin amyloid-like fibrils formed with ultrasound treatment and chitosan: A potential vehicle for betanin and curcumin with improved chemical stability and slow release in vitro

IF 11 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids Pub Date : 2025-02-24 DOI:10.1016/j.foodhyd.2025.111272

Qin Yang , Xiao-Hong Yu , Jing-Wen Zhang , Yi-Xuan Jiang , Han-Qing Chen

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引用次数: 0

Abstract

Plant protein amyloid-like fibrils have aroused great attention as potential vehicles for bioactive substances. In this study, arachin amyloid-like fibrils (AF) formed with ultrasound treatment (UAF) during the lag phase of fibrillation process were prepared and complexed with chitosan (CS) to construct novel vehicles for betanin (Bet) and curcumin (Cur). The aim of this work was to characterize the AF-CS and UAF-CS complexes, the combination mechanism of the binary complexes with Bet/Cur and their protection effect on Bet and Cur. Results showed that combination with CS decreased the thioflavin T fluorescence intensity, surface hydrophobicity and surface charges of AF and UAF. When the AF-CS and UAF-CS combined with Bet or Cur, the ternary complexes showed intricate network structures as visualized by atomic force microscopy. Moreover, the AF-CS-Cur and UAF-CS-Cur showed larger network than AF-CS-Bet and UAF-CS-Bet. The fluorescence quenching and time‒resolved fluorescence results revealed static quenching of AF-CS and UAF-CS by Bet or Cur. Compared with AF-CS, UAF-CS showed higher values of quenching constant (Ksv = 1.9948 × 10⁵ mol⁻¹ L, 2.7240 × 10⁵ mol⁻¹ L) and binding constant (Ka = 156.2428 mol⁻¹ L, 42717 mol⁻¹ L) to Bet and Cur, respectively. Moreover, the retention rate of Bet and Cur in the UAF-CS complex (57.6%, 91.0%) was higher than those in the AF-CS complex (47.7%, 87.2%) after heating for 40 min, respectively. Additionally, more content of Bet and Cur were retained in UAF-CS complex when they were exposed to NaCl (100–400 mM). The Bet and Cur in UAF-CS complex also showed sustained release behavior in the simulated gastrointestinal digestion. This study indicates that the vehicle of UAF-CS complex could effectively stabilize Bet and Cur, which can promote the application of Bet and Cur in fat-free functional beverages.

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来源期刊

Food Hydrocolloids 工程技术-食品科技

CiteScore

19.90

自引率

14.00%

发文量

871

审稿时长

37 days

期刊介绍： Food Hydrocolloids publishes original and innovative research focused on the characterization, functional properties, and applications of hydrocolloid materials used in food products. These hydrocolloids, defined as polysaccharides and proteins of commercial importance, are added to control aspects such as texture, stability, rheology, and sensory properties. The research's primary emphasis should be on the hydrocolloids themselves, with thorough descriptions of their source, nature, and physicochemical characteristics. Manuscripts are expected to clearly outline specific aims and objectives, include a fundamental discussion of research findings at the molecular level, and address the significance of the results. Studies on hydrocolloids in complex formulations should concentrate on their overall properties and mechanisms of action, while simple formulation development studies may not be considered for publication. The main areas of interest are: -Chemical and physicochemical characterisation Thermal properties including glass transitions and conformational changes- Rheological properties including viscosity, viscoelastic properties and gelation behaviour- The influence on organoleptic properties- Interfacial properties including stabilisation of dispersions, emulsions and foams- Film forming properties with application to edible films and active packaging- Encapsulation and controlled release of active compounds- The influence on health including their role as dietary fibre- Manipulation of hydrocolloid structure and functionality through chemical, biochemical and physical processes- New hydrocolloids and hydrocolloid sources of commercial potential. The Journal also publishes Review articles that provide an overview of the latest developments in topics of specific interest to researchers in this field of activity.