Identification a novel syntaxin-like protein from silkworm Bombyx mori pathogen Nosema bombycis and characteristics its membrane fusion function

IF 3.6 3区 生物学 Q1 ZOOLOGY
Liuyi Hong , Qing Lv , Hongjie Liao , Zhengkai Xie , Maoping Shao , Yuanyu Zhang , Yuqing Chen , Guoqing Pan , Mengxian Long , Zeyang Zhou
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Abstract

Pebrine is a serious disease of the silkworm, Bombyx mori, caused by the first identified microsporidium Nosema bombycis, which is an obligate parasitic single-celled eukaryote. The pathogen can spread both horizontally and vertically, severely affecting sericulture. SNARE proteins mainly mediate the transport of vesicles and membrane fusion, playing a key role in the biological processes. The microsporidium is known to have a well-developed membrane system, especially the polaroplast which occupies most of the volume of mature spores. In order to explore the function of microsporidian SNARE protein, the transcription and subcellular localization characteristics of a novel Syntaxin-like protein (NbSTX-like) from N. bombycis that had a conserved t-SNARE motif were analyzed. In the different development stages of N. bombycis, the NbSTX-like expressed in the nucleus of meronts, then transited to the cytoplasm in the sporonts, gradually gathered at the two ends of the sporoblasts, and finally concentrated at the polaroplast, posterior vacuole and plasma membrane region of mature spores. Interestingly, the rNbSTX-like protein could fuse liposomes to form large vesicular and tubular structures. The formation of sporoplasms was inhibited by the anti-NbSTX-like serum, implying that NbSTX-like protein participated in sporoplasm maturation. These findings laid a foundation for studying the function of SNARE proteins in microsporidia and provided new insights for the prevention and control of sericulture pathogens.

Abstract Image

家蚕病原菌家蚕微虫一种新的合成素样蛋白的鉴定及其膜融合功能特征
微孢子虫病是家蚕的一种严重疾病,由首次发现的专性寄生单细胞真核生物微孢子虫引起。病原菌可横向和纵向传播,严重影响养蚕业。SNARE蛋白主要介导囊泡运输和膜融合,在生物过程中起关键作用。众所周知,微孢子虫具有发育良好的膜系统,尤其是极质体,它占据了成熟孢子的大部分体积。为了探究微孢子虫SNARE蛋白的功能,我们分析了一种具有保守t-SNARE基序的新Syntaxin-like蛋白(NbSTX-like)的转录和亚细胞定位特征。在家蚕不同发育阶段,NbSTX-like在孢子核中表达,然后转移到孢子体的细胞质中,逐渐聚集在成孢子细胞的两端,最后集中在成熟孢子的极质体、后液泡和质膜区。有趣的是,rnbstx样蛋白可以融合脂质体形成大的囊泡和管状结构。抗nbstx样血清抑制了孢子质形成,提示nbstx样蛋白参与了孢子质成熟过程。这些发现为研究SNARE蛋白在微孢子虫中的功能奠定了基础,并为蚕桑病原菌的防治提供了新的思路。
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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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