Evaluation of potential anti-inflammatory activities in crude, clean, and fractionated extracts of rosmarinic acid and eupatorin from Orthosiphon aristatus (Blume) Miq

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-02-20 DOI:10.1016/j.bcab.2025.103537

Salfarina Ezrina Mohmad Saberi , Lee Suan Chua

{"title":"Evaluation of potential anti-inflammatory activities in crude, clean, and fractionated extracts of rosmarinic acid and eupatorin from Orthosiphon aristatus (Blume) Miq","authors":"Salfarina Ezrina Mohmad Saberi , Lee Suan Chua","doi":"10.1016/j.bcab.2025.103537","DOIUrl":null,"url":null,"abstract":"<div><div><em>Orthosiphon aristatus</em> has been demonstrated to possess natural anti-inflammatory properties. This study aims to concentrate rosmarinic acid (RosA) and eupatorin (EUP) content in the plant extracts using different ethanol percentages and to evaluate anti-inflammatory potential of RosA and EUP extracts. Reflux extraction of <em>O. aristatus</em> was conducted using different ethanol concentrations ranging from 60 % to 100 %. The results found that 60 % ethanol produced the highest yield of crude extract, while the highest yields of clean and fraction extracts were from 90 % ethanol. The optimal yield of RosA was in 80 % ethanol crude extract, and 90 % ethanol clean and fraction extracts. However, the highest yield of EUP was obtained from 60 % ethanolic fraction extract, whereas 100 % ethanol was optimal for crude and clean extracts. A substantial amount of EUP in 60_Fr exhibited the most effective inhibition of lipoxygenase (LOX), with an inhibition concentration of 27.91 μg/mL. Conversely, sample 100_Cr characterized by a high concentration of RosA, demonstrated the highest inhibitory effect of α-amylase, with an inhibition value of 100.41 μg/mL. Ethanol concentration played an important role to concentrate both bioactive compounds in the plant extracts. Different ethanol concentrations would recover varying levels of compounds in the crude, clean and fraction extracts to relate to their changes in anti-inflammatory properties. Concentrating EUP enhanced the LOX inhibition, whereas concentrating RosA promoted α-amylase inhibition. The findings of this study are critical for identifying therapeutic compounds in different forms of <em>O. aristatus</em> extracts to treat inflammatory-induced illnesses.</div></div>","PeriodicalId":8774,"journal":{"name":"Biocatalysis and agricultural biotechnology","volume":"65 ","pages":"Article 103537"},"PeriodicalIF":3.4000,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biocatalysis and agricultural biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878818125000507","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Orthosiphon aristatus has been demonstrated to possess natural anti-inflammatory properties. This study aims to concentrate rosmarinic acid (RosA) and eupatorin (EUP) content in the plant extracts using different ethanol percentages and to evaluate anti-inflammatory potential of RosA and EUP extracts. Reflux extraction of O. aristatus was conducted using different ethanol concentrations ranging from 60 % to 100 %. The results found that 60 % ethanol produced the highest yield of crude extract, while the highest yields of clean and fraction extracts were from 90 % ethanol. The optimal yield of RosA was in 80 % ethanol crude extract, and 90 % ethanol clean and fraction extracts. However, the highest yield of EUP was obtained from 60 % ethanolic fraction extract, whereas 100 % ethanol was optimal for crude and clean extracts. A substantial amount of EUP in 60_Fr exhibited the most effective inhibition of lipoxygenase (LOX), with an inhibition concentration of 27.91 μg/mL. Conversely, sample 100_Cr characterized by a high concentration of RosA, demonstrated the highest inhibitory effect of α-amylase, with an inhibition value of 100.41 μg/mL. Ethanol concentration played an important role to concentrate both bioactive compounds in the plant extracts. Different ethanol concentrations would recover varying levels of compounds in the crude, clean and fraction extracts to relate to their changes in anti-inflammatory properties. Concentrating EUP enhanced the LOX inhibition, whereas concentrating RosA promoted α-amylase inhibition. The findings of this study are critical for identifying therapeutic compounds in different forms of O. aristatus extracts to treat inflammatory-induced illnesses.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.