Correlation between SNPs in promoter region of gene, L-Type lectin (LvLTLC1) and the resistance trait of shrimp, Litopenaeus vannamei, against vibrios

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-03-01 DOI:10.1016/j.aqrep.2025.102725

Shuyang Wen , Lihong Yuan , Jiayue Yin , Chaoqun Hu , Xin Zhang , Yunyi Huang , Chujie Zhao , Liyan Liu , Peng Luo

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引用次数: 0

Abstract

Pacific white shrimp, Litopenaeus vannamei (L. vannamei), is the most economically valuable shrimp species in world. Bacterial diseases mainly caused by various vibrios bring huge economic loss in shrimp aquaculture. Previous research has confirmed that LvLTLC1 gene coding a L-type lectin of L. vannamei plays a pivotal role in the resistance of shrimp against pathogenic vibrios, which implies the potential of LvLTLC1 as a target gene to develop SNP markers for genetic breeding of the shrimp. In this study, pan-vibrio resistance (PVR) trait of L. vannamei was quantitatively evaluated by vibrios loads in hepatopancreas and shrimp individuals were divided into vibrios-resistance (VR) groups and vibrios-susceptible (VS) groups according to their vibrios-resistance performance (VRP). Expression assay of LvLTLC1 showed that mRNA levels of LvLTLC1 in the hepatopancreas of the VR group was significantly higher than that in the VS groups. Totally, 24 SNPs were identified in the promoter regions of LvLTLC1 in shrimp individuals. Correlation analysis between the genotypes of these single nucleotide polymorphism (SNPs) and the VRP revealed that a homozygous GG genotype in SNP8 (g.–256 A/G) were significantly correlated with the VRP (P < 0.05). Linkage disequilibrium (LD) analysis revealed a high linkage block of SNP9 (g.–249 T/G) and SNP10 (g.–235 A/G), and a TT/GG combined genotype of SNP9 and SNP10 was further discovered to be significantly associated with strong VRP (P < 0.05). Furthermore, the variations in motifs caused by SNP8, SNP9, and SNP10 led to changes of binding patterns of transcription factors. The present study first revealed the presence of abundant SNPs in a gene promoter region of shrimp L. vannamei and found that specific genotypes of SNPs in LvLTLC1 promoter confer strong VRP in the shrimp. The homozygous GG genotype in SNP8 and the TT/GG genotype combination of SNP9 and SNP10 can be served as two potential molecular markers for the PVR trait in genetic breeding of L. vannamei.

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.