YAP1 regulates endometrial receptivity by promoting the plasma membrane transformation and proliferation of bovine endometrial epithelial cells

IF 2.4 2区农林科学 Q3 REPRODUCTIVE BIOLOGY

Theriogenology Pub Date : 2025-02-22 DOI:10.1016/j.theriogenology.2025.02.017

Han Zhou, Jinxin Zhang, Wen Feng, Nuoer Chen, Talha Umar, Xinyu Feng, Wenjing Liu, Changwei Qiu, Ganzhen Deng

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引用次数: 0

Abstract

Plasma membrane transformation (PMT) during early pregnancy (EP) is a widely observed phenomenon across species, but its role in cows remains understudied. Yes1-associated transcriptional regulator (YAP1) plays a vital role in human embryo implantation and uterine development. However, its relationship with PMT in bovine endometrium remains unclear. This study aims to explore the potential influence of YAP1 on PMT and the proliferation of endometrial epithelial cells. We observed PMT-related changes in the endometrium of cows during EP, characterized by reduced morphometry scores and increased fully developed pinopodes. The epithelial marker cadherin-1 (CDH1, also known as E-cadherin) expression decreased, while the mesenchymal marker vimentin (VIM) expression increased. Additionally, YAP1 expression was up-regulated and activated in the endometrial epithelium during EP. Interferon-tau (IFNT) potentially promoted PMT-related changes and activated YAP1. The knockdown of YAP1 diminished the effect of IFNT on PMT. At the same time, overexpression of YAP1 might enhance PMT-related changes and promote the proliferation, migration, and invasion ability of bovine endometrial epithelial cells (bEECs). Our findings suggest that YAP1 is activated during EP and may regulate endometrial receptivity by promoting PMT and cell proliferation.

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来源期刊

Theriogenology 农林科学-生殖生物学

CiteScore

5.50

自引率

14.30%

发文量

387

审稿时长

72 days

期刊介绍： Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.