The genomic characteristics of dominant Salmonella enterica serovars from retail pork in Sichuan province, China

IF 5 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Lanqi Li , Honghu Sun , Jianyun Zhao , Huanjing Sheng , Menghan Li , Lanxin Zhao , Shiwei Liu , Séamus Fanning , Lu Wang , Yang Wang , Yongning Wu , Hao Ding , Li Bai
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引用次数: 0

Abstract

Foodborne Salmonella is the main cause of salmonellosis in China. Porcine animals are a reservoir for this bacterium consequently posing a threat to food safety and public health. In this study, 157 out of 240 pork samples (65.42 %) were identified as Salmonella-positive. From these, after isolation and deduplication, 376 Salmonella isolates were collected. Twenty four serovars were identified based on WGS, among which S. London/ST155 (24.47 %), S. Rissen/ST469 (23.40 %), S. Derby/ST40 (13.56 %), and S. 4,[5],12:i:- (monophasic S. Typhimurium)/ST34 (13.30 %) were dominant. In all, 69.68 % (262/376) of these isolates expressed multidrug resistance (MDR, defined as resistance to compounds in three or more antimicrobial classes) phenotypes with S. London (54.35 %, 50/92) accounting for the highest proportion of these. Notably, the resistance to front-line critically important antimicrobial agents (CIA), including cephalosporins, ciprofloxacin, and azithromycin was 0.80 %. Based on in silico analysis, antimicrobial resistant-encoding genes (ARG) identified in the MDR isolates included aac(3)-IId, aac(6′)-Iaa, blaTEM-1B, mph(A), qnrB6, aac(6′)-Ib-cr, sul1, sul2, and tet(A), which expressed resistance to aminoglycosides, β-lactams, macrolides, quinolones, sulfonamides, and tetracyclines. Furthermore, diverse biocide and heavy metal resistance-encoding genes were distributed across different serovars with triC encoding triclosan resistance being identified exclusively in S. London. Moreover, monophasic S. 4,[5],12:i:- carried the greatest number of virulence factors and heavy metal resistance genes among the dominant serovars. This study extended our understanding of the genomic epidemiology and multidrug resistance of Salmonella derived from pork and highlighted the potential risk to human health, posed by commonly encountered serovars.
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来源期刊
International journal of food microbiology
International journal of food microbiology 工程技术-食品科技
CiteScore
10.40
自引率
5.60%
发文量
322
审稿时长
65 days
期刊介绍: The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.
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