The genomic characteristics of dominant Salmonella enterica serovars from retail pork in Sichuan province, China

IF 5 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Lanqi Li , Honghu Sun , Jianyun Zhao , Huanjing Sheng , Menghan Li , Lanxin Zhao , Shiwei Liu , Séamus Fanning , Lu Wang , Yang Wang , Yongning Wu , Hao Ding , Li Bai
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引用次数: 0

Abstract

Foodborne Salmonella is the main cause of salmonellosis in China. Porcine animals are a reservoir for this bacterium consequently posing a threat to food safety and public health. In this study, 157 out of 240 pork samples (65.42 %) were identified as Salmonella-positive. From these, after isolation and deduplication, 376 Salmonella isolates were collected. Twenty four serovars were identified based on WGS, among which S. London/ST155 (24.47 %), S. Rissen/ST469 (23.40 %), S. Derby/ST40 (13.56 %), and S. 4,[5],12:i:- (monophasic S. Typhimurium)/ST34 (13.30 %) were dominant. In all, 69.68 % (262/376) of these isolates expressed multidrug resistance (MDR, defined as resistance to compounds in three or more antimicrobial classes) phenotypes with S. London (54.35 %, 50/92) accounting for the highest proportion of these. Notably, the resistance to front-line critically important antimicrobial agents (CIA), including cephalosporins, ciprofloxacin, and azithromycin was 0.80 %. Based on in silico analysis, antimicrobial resistant-encoding genes (ARG) identified in the MDR isolates included aac(3)-IId, aac(6′)-Iaa, blaTEM-1B, mph(A), qnrB6, aac(6′)-Ib-cr, sul1, sul2, and tet(A), which expressed resistance to aminoglycosides, β-lactams, macrolides, quinolones, sulfonamides, and tetracyclines. Furthermore, diverse biocide and heavy metal resistance-encoding genes were distributed across different serovars with triC encoding triclosan resistance being identified exclusively in S. London. Moreover, monophasic S. 4,[5],12:i:- carried the greatest number of virulence factors and heavy metal resistance genes among the dominant serovars. This study extended our understanding of the genomic epidemiology and multidrug resistance of Salmonella derived from pork and highlighted the potential risk to human health, posed by commonly encountered serovars.
四川省零售猪肉中优势肠沙门氏菌血清型的基因组特征
食源性沙门氏菌是中国沙门氏菌病的主要病因。猪是这种细菌的宿主,因此对食品安全和公众健康构成威胁。在本研究中,240份猪肉样品中有157份(65.42%)被鉴定为沙门氏菌阳性。从这些样本中,经过分离和重复分析,共分离出376株沙门氏菌。WGS共鉴定出24种血清型,其中以S. London/ST155(24.47%)、S. Rissen/ST469(23.40%)、S. Derby/ST40(13.56%)和S. 4、[5]、12:i:-(单相鼠伤寒链球菌)/ST34(13.30%)为主。其中,69.68%(262/376)的分离株表达多药耐药表型(MDR,定义为对三种或三种以上抗菌药物的化合物耐药),其中伦敦链球菌(54.35%,50/92)的比例最高。值得注意的是,对一线重要抗菌药物(CIA)的耐药性为0.80%,包括头孢菌素、环丙沙星和阿奇霉素。基于硅基分析,在MDR分离株中鉴定出耐药编码基因(ARG)包括aac(3)-IId、aac(6’)-Iaa、blatemm - 1b、mph(A)、qnrB6、aac(6’)-Ib-cr、sul1、sul2和tet(A),这些基因表达对氨基糖苷类、β-内酰胺类、大环内酯类、喹诺酮类、磺胺类和四环素类药物的耐药。此外,不同的杀菌剂和重金属抗性编码基因分布在不同的血清型中,其中trc编码三氯生抗性仅在S. London中被鉴定出来。此外,在优势血清型中,单相S. 4、[5]、12:i:-携带的毒力因子和重金属抗性基因数量最多。这项研究扩展了我们对来自猪肉的沙门氏菌的基因组流行病学和多药耐药性的理解,并强调了常见的血清型沙门氏菌对人类健康的潜在风险。
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来源期刊
International journal of food microbiology
International journal of food microbiology 工程技术-食品科技
CiteScore
10.40
自引率
5.60%
发文量
322
审稿时长
65 days
期刊介绍: The International Journal of Food Microbiology publishes papers dealing with all aspects of food microbiology. Articles must present information that is novel, has high impact and interest, and is of high scientific quality. They should provide scientific or technological advancement in the specific field of interest of the journal and enhance its strong international reputation. Preliminary or confirmatory results as well as contributions not strictly related to food microbiology will not be considered for publication.
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