{"title":"The isolation, identification, whole-genome sequencing of Trichoderma brevicompactum TB2 and its effects on plant growth-promotion","authors":"Hao Li, Zuohereguli Kuerban, Rou Jiang, Feixue He, Xiao Hu, Yangchun Xu, Caixia Dong, Qirong Shen","doi":"10.1007/s11104-025-07304-x","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Aims</h3><p><i>Trichoderma</i> species are recognized for their plant growth-promoting and biocontrol properties. This study aimed to isolate effective <i>Trichoderma</i> strains from the rhizosphere of pear (<i>Pyrus betulifolia</i> Bunge) trees and evaluate their growth-promoting effects on various plant species.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Ten <i>Trichoderma</i> strains were isolated using a gradient dilution plating method, and <i>Trichoderma brevicompactum</i> TB2 was selected through laboratory and greenhouse experiments. The growth-promoting properties of TB2 were assessed in pot experiments with cucumber (<i>Cucumis sativus</i> L.) and apple rootstock (<i>Malus hupehensis</i> Rehd) seedlings. Whole-genome sequencing was performed to annotate TB2 genes across various databases and predict secondary metabolite biosynthetic gene clusters. Production of indole-3-acetic acid (IAA), siderophores, and iron-solubilizing capabilities were measured.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>TB2 showed high production of IAA, siderophores, and iron-solubilizing abilities. The draft genome, spanning 18 scaffolds totaling 38.70 Mb with 10,436 protein-coding genes, revealed 431 genes encoding carbohydrate-active enzymes involved in metabolic pathways. Application of TB2 spore suspension (1.0 × 10⁶ CFU/g soil) significantly increased fresh and dry weights across all tested plants and enhanced root development metrics in <i>C. sativus</i> and <i>P. betulifolia</i> seedlings. TB2 inoculation also boosted major and minor nutrient levels in the leaves of <i>M. hupehensis</i> seedlings.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>TB2 exhibits strong growth-promoting effects across different plant species, improving physiological indicators such as plant height, stem diameter, and biomass, while enhancing nutrient absorption without host specificity. TB2 can thus be utilized as an excellent agricultural microbial resource strain.</p>","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"52 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-025-07304-x","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Aims
Trichoderma species are recognized for their plant growth-promoting and biocontrol properties. This study aimed to isolate effective Trichoderma strains from the rhizosphere of pear (Pyrus betulifolia Bunge) trees and evaluate their growth-promoting effects on various plant species.
Methods
Ten Trichoderma strains were isolated using a gradient dilution plating method, and Trichoderma brevicompactum TB2 was selected through laboratory and greenhouse experiments. The growth-promoting properties of TB2 were assessed in pot experiments with cucumber (Cucumis sativus L.) and apple rootstock (Malus hupehensis Rehd) seedlings. Whole-genome sequencing was performed to annotate TB2 genes across various databases and predict secondary metabolite biosynthetic gene clusters. Production of indole-3-acetic acid (IAA), siderophores, and iron-solubilizing capabilities were measured.
Results
TB2 showed high production of IAA, siderophores, and iron-solubilizing abilities. The draft genome, spanning 18 scaffolds totaling 38.70 Mb with 10,436 protein-coding genes, revealed 431 genes encoding carbohydrate-active enzymes involved in metabolic pathways. Application of TB2 spore suspension (1.0 × 10⁶ CFU/g soil) significantly increased fresh and dry weights across all tested plants and enhanced root development metrics in C. sativus and P. betulifolia seedlings. TB2 inoculation also boosted major and minor nutrient levels in the leaves of M. hupehensis seedlings.
Conclusions
TB2 exhibits strong growth-promoting effects across different plant species, improving physiological indicators such as plant height, stem diameter, and biomass, while enhancing nutrient absorption without host specificity. TB2 can thus be utilized as an excellent agricultural microbial resource strain.
期刊介绍：
Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.
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