Combined Treatment of Metformin and Resveratrol Promotes Myogenesis Through Increased Irisin Release in C2C12 Cells.

IF 3.5 3区医学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Pharmaceutical Research Pub Date : 2025-03-01 Epub Date: 2025-02-26 DOI:10.1007/s11095-025-03834-2

Eun Ji Seong, Yejin Kim, Zheng-Yuan Su, Hee-Taik Kang, Jong Hun Lee

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引用次数: 0

Abstract

Purpose: This study aimed to investigate the additive effects of a combination of metformin and resveratrol on irisin expression in C2C12 cells.

Methods: The study involved treating C2C12 cells with metformin and resveratrol, either alone or in combination, and analyzing their effects on myogenesis and irisin release. The activation of signaling pathways, including AMPK/SIRT1/PGC1α, as well as the relative mRNA and protein expression levels of MyoD, myogenin, and Myh were also assessed.

Results: Combination treatment with metformin and resveratrol significantly increased MyoD, myogenin, Myh, and FNDC5 expression compared with the group treated with metformin alone. The increase in irisin production was associated with phosphorylation of AMPK and upregulation of PGC-1α and SIRT1, indicating activation of the AMPK/SIRT1/PGC-1α pathway. The mRNA and protein expression levels of MyoD, myogenin, and Myh were also significantly higher in the combination treatment group compared to the metformin alone group.

Conclusion: The combination of metformin and resveratrol effectively increased irisin release through the AMPK/Sirt1/PGC-1α pathway, suggesting that this combination treatment could enhance myogenesis.

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二甲双胍和白藜芦醇联合治疗通过增加鸢尾素在C2C12细胞中的释放促进肌肉生成。

目的：探讨二甲双胍联合白藜芦醇对C2C12细胞中鸢尾素表达的加性作用。方法：研究二甲双胍和白藜芦醇单独或联合作用于C2C12细胞，分析其对肌肉生成和鸢尾素释放的影响。我们还评估了AMPK/SIRT1/PGC1α等信号通路的激活情况，以及MyoD、myogenin和Myh的相对mRNA和蛋白表达水平。结果：与单用二甲双胍组比较，二甲双胍联合白藜芦醇组MyoD、myogenin、Myh、FNDC5表达明显升高。鸢尾素产量的增加与AMPK的磷酸化以及PGC-1α和SIRT1的上调有关，表明AMPK/SIRT1/PGC-1α途径被激活。与单用二甲双胍组相比，联合治疗组MyoD、myogenin、Myh mRNA和蛋白表达水平也显著升高。结论：二甲双胍与白藜芦醇联用可通过AMPK/Sirt1/PGC-1α途径有效增加鸢尾素的释放，提示联用可促进肌肉生成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical Research 医学-化学综合

CiteScore

6.60

自引率

5.40%

发文量

276

审稿时长

3.4 months

期刊介绍： Pharmaceutical Research, an official journal of the American Association of Pharmaceutical Scientists, is committed to publishing novel research that is mechanism-based, hypothesis-driven and addresses significant issues in drug discovery, development and regulation. Current areas of interest include, but are not limited to: -(pre)formulation engineering and processing- computational biopharmaceutics- drug delivery and targeting- molecular biopharmaceutics and drug disposition (including cellular and molecular pharmacology)- pharmacokinetics, pharmacodynamics and pharmacogenetics. Research may involve nonclinical and clinical studies, and utilize both in vitro and in vivo approaches. Studies on small drug molecules, pharmaceutical solid materials (including biomaterials, polymers and nanoparticles) biotechnology products (including genes, peptides, proteins and vaccines), and genetically engineered cells are welcome.