Should we Cryopreserve Allogenic Hematopoietic Stem Cell Grafts?

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

In vivo Pub Date : 2025-03-01 DOI:10.21873/invivo.13890

Ewa Bembnista, Patrycja Stawicka, Paula Matuszak, Anna Łojko-Dankowska, Dominik Dytfeld, Magdalena Matuszak, Anna Wache, Katarzyna Kaźmierska, Ewa Majewska, Krzysztof Lewandowski, Lidia Gil

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引用次数: 0

Abstract

Background/aim: Cryopreservation of allogeneic hematopoietic cells can be an necessary step in the preparation of a graft for transplantation. The purpose of this study was to evaluate the safety of cryopreservation of allogeneic hematopoietic cells and the clinical outcomes of patients who received a cryopreserved transplant.

Patients and methods: The study included data from a comparative analysis conducted on a group of 100 patients diagnosed with acute myeloid leukemia who received cryopreserved (50 patients) and fresh (50 patients) transplants. Both quantitative and qualitative parameters of the grafts were evaluated, as well as the patients' hematopoietic recovery.

Results: Statistical analysis of the time to engraftment of neutrophils, nucleated cells, and platelets showed no differences between the group of patients who received cryopreserved transplants and those who received fresh transplants. A higher count of CD34+ cells in the graft significantly reduced the time required for patients to reach their reference values of neutrophils, nuclear cells, and platelets (p<0.05).

Conclusion: Cryopreservation of allogenic transplants should be reserved as an option when fresh grafts are not feasible for various reasons.

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同种异体造血干细胞移植是否应该冷冻保存？

背景/目的：异体造血细胞的低温保存是制备移植用移植物的必要步骤。本研究的目的是评估异体造血细胞冷冻保存的安全性和接受冷冻保存移植的患者的临床结果。患者和方法：该研究包括对100名诊断为急性髓性白血病的患者进行比较分析的数据，这些患者接受了冷冻保存（50例）和新鲜移植（50例）。评估移植物的定量和定性参数，以及患者的造血功能恢复情况。结果：对中性粒细胞、有核细胞、血小板的移植时间进行统计分析，结果显示低温保存移植组与新鲜移植组之间无差异。移植物中较高的CD34+细胞计数显著缩短了患者达到中性粒细胞、核细胞和血小板参考值所需的时间(结论：当由于各种原因无法进行新鲜移植物时，应保留同种异体移植物的冷冻保存。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.