Secretome from HMGB1 Box A-over-expressing Adipose-derived Stem Cells Shows Potential for Skin Rejuvenation by Senescence Reversal in PM2.5-induced Senescence Cells via Stem Cell Induction.

IF 1.8 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

In vivo Pub Date : 2025-03-01 DOI:10.21873/invivo.13881

Zin Zin Ei, Apiwat Mutirangura, Pithi Chanvorachote

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引用次数: 0

Abstract

Background/aim: Exposure to particulate matter 2.5 (PM2.5) can lead to cellular senescence by generating reactive oxygen species (ROS). Box A, a DNA-binding domain found in HMGB1, is known for its ability to counteract aging characteristics. This study explored whether BoxA-induced adipose-derived stem cells secretome (BoxA-SC) can reverse senescence in DP and HWPc cells.

Materials and methods: The stemness characteristics and reversal of senescence by BoxA-SC in PM2.5-induced DP and HWPc cells were assessed at the mRNA level using RT-qPCR and at the protein level using immunofluorescence analysis.

Results: BoxA-SC (1:20) treatment for 48 h induced stemness and reversed PM2.5-induced cell senescence in DP and HWPc cells. BoxA-SC significantly reduced senescence markers, including SA-β-gal staining, and decreased mRNA levels of senescence-associated secretory phenotype factors (IL1α, IL7, CXCL1) in PM2.5-induced senescent cells. DP and HWPc cells exposed to PM2.5 exhibited an increase in p21 and p16 mRNA and protein levels, which was reversed by BoxA-SC. BoxA-SC reduced p21 and p16 in DP senescent cells approximately 3- and 2-fold, respectively, compared to untreated senescent cells.

Conclusion: BoxA-SC can potentially reverse cellular senescence, highlighting the therapeutic potential of stem cells in skin rejuvenation and anti-aging treatments.

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背景/目的：暴露于颗粒物 2.5（PM2.5）可通过产生活性氧（ROS）导致细胞衰老。Box A是HMGB1中的一个DNA结合域，因其能够抵消衰老特征而闻名。本研究探讨了BoxA诱导的脂肪源性干细胞分泌组（BoxA-SC）能否逆转DP和HWPc细胞的衰老：采用RT-qPCR在mRNA水平和免疫荧光分析在蛋白质水平评估BoxA-SC在PM2.5诱导的DP和HWPc细胞中的干性特征和逆转衰老的作用：结果：BoxA-SC（1:20）处理DP和HWPc细胞48小时可诱导干性并逆转PM2.5诱导的细胞衰老。在PM2.5诱导的衰老细胞中，BoxA-SC能明显减少衰老标记物，包括SA-β-gal染色，并降低衰老相关分泌表型因子（IL1α、IL7、CXCL1）的mRNA水平。暴露于 PM2.5 的 DP 和 HWPc 细胞表现出 p21 和 p16 mRNA 和蛋白质水平的增加，而 BoxA-SC 逆转了这种增加。与未经处理的衰老细胞相比，BoxA-SC 可使 DP 衰老细胞中的 p21 和 p16 分别降低约 3 倍和 2 倍：结论：BoxA-SC有可能逆转细胞衰老，凸显了干细胞在皮肤年轻化和抗衰老治疗中的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

In vivo 医学-医学：研究与实验

CiteScore

4.20

自引率

4.30%

发文量

330

审稿时长

3-8 weeks

期刊介绍： IN VIVO is an international peer-reviewed journal designed to bring together original high quality works and reviews on experimental and clinical biomedical research within the frames of physiology, pathology and disease management. The topics of IN VIVO include: 1. Experimental development and application of new diagnostic and therapeutic procedures; 2. Pharmacological and toxicological evaluation of new drugs, drug combinations and drug delivery systems; 3. Clinical trials; 4. Development and characterization of models of biomedical research; 5. Cancer diagnosis and treatment; 6. Immunotherapy and vaccines; 7. Radiotherapy, Imaging; 8. Tissue engineering, Regenerative medicine; 9. Carcinogenesis.