Antidiabetic potential of vanadium complexes combined with olive leaf extracts: a viable approach to reduce metal toxicity

IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Daniele Sanna, Angela Fadda, Milena Casula, Grazia Palomba, Maria Cristina Sini, Maria Colombino, Carla Rozzo, Giuseppe Palmieri, Carmela Gallo, Dalila Carbone, Laura Siracusa, Luana Pulvirenti, Valeria Ugone
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Abstract

Vanadium compounds are known for their antidiabetic properties due to their ability to interfere with numerous mechanisms that lead to the decrease of blood glucose levels. Although some of these compounds have reached clinical trials and have the advantage of being orally administrable, no vanadium-containing drugs are currently available on the market, primarily due to the high doses required, which can lead intestinal and renal problems in case of long-term treatments. In this study, plant extracts obtained from olive leaves (Olea europaea L.) were combined with vanadium complexes with established antidiabetic activity with the aim of reducing their metal toxicity and, at the same time, amplifying their hypoglycemic action. The extracts were characterized by chromatographic and spectroscopic methods showing a composition rich in polyphenols and a high antioxidant activity. Formulations containing a vanadium complex (bis(maltolato)oxidovanadium(IV), BMOV, or bis(picolinato)oxidovanadium(IV), BPOV) mixed with different amount of olive leaves extract were tested in vitro to evaluate intestinal toxicity and hypoglycemic activity. The results demonstrated that the plant extracts are generally non-toxic toward human colon fibroblast in the whole range of tested concentrations and some of them are particularly effective in reducing the toxicity of the two vanadium compounds. Further in vitro tests conducted on differentiated human adipocyte cell lines revealed a significant increase in glucose uptake following treatment with the mixed formulations, compared to the effect of the individual components, indicating a synergistic effect. Immunocytochemical assays suggested that the translocation of GLUT4 transporter can be involved in the mechanism of action.

钒配合物与橄榄叶提取物联合的抗糖尿病潜力:一种降低金属毒性的可行方法。
钒化合物因其抗糖尿病特性而闻名,因为它们能够干扰许多导致血糖水平降低的机制。虽然其中一些化合物已进入临床试验,并具有可口服的优点,但目前市场上还没有含钒的药物,主要原因是所需剂量大,在长期治疗的情况下可能导致肠道和肾脏问题。在这项研究中,从橄榄叶中提取的植物提取物(Olea europaea L.)与具有抗糖尿病活性的钒配合物结合,目的是降低其金属毒性,同时增强其降糖作用。通过色谱和光谱方法对提取物进行了表征,发现其成分中含有丰富的多酚类物质,具有较高的抗氧化活性。将含钒络合物(双(麦芽糖)氧化钒(IV), BMOV,或双(picolinato)氧化钒(IV), BPOV)与不同量的橄榄叶提取物混合的配方进行体外测试,以评估肠道毒性和降血糖活性。结果表明,在整个测试浓度范围内,植物提取物对人结肠成纤维细胞总体无毒,其中部分提取物对降低两种钒化合物的毒性特别有效。对分化的人类脂肪细胞系进行的进一步体外试验显示,与单独成分的效果相比,使用混合配方治疗后,葡萄糖摄取显著增加,表明具有协同作用。免疫细胞化学分析提示,GLUT4转运蛋白的易位可能参与了其作用机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biometals
Biometals 生物-生化与分子生物学
CiteScore
5.90
自引率
8.60%
发文量
111
审稿时长
3 months
期刊介绍: BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.
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