Comparison of Immune Responses Induced in Gibel Carp, Carassius auratus gibelio Immersion, and Intraperitoneal Vaccinated With Live Attenuated Cyprinid Herpesvirus 2 (CyHV-2)

IF 1.9 4区 农林科学 Q2 FISHERIES
Jia Wang, Weijie Zhang, Jizhuo Liu, Qiang Li, Zhitao Qi, Chang Wei
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Abstract

This study investigated the immune responses induced in gibel carp, Carassius auratus gibelio, following immersion and intraperitoneal (i.p.) vaccination with live attenuated Cyprinid herpesvirus 2 (CyHV-2). Transcriptome sequencing analysis was performed on the kidney tissues of the experimental fish at 3, 7, and 14 days postvaccination. A staggering 948 million readings were assembled into 96,891 genes, with an average length of 1214 bp. We analyzed the KEGG enrichment, differentially expressed genes (DEGs), and DEGs temporal expression patterns between the immersion and i.p. injection groups. Our findings revealed that both i.p. injection and immersion vaccination significantly altered immune-related genes in gibel carp, suggesting that the prepared attenuated vaccine effectively stimulates the fish’s immune response. Generally, the injection group exhibited higher gene expression levels in the kidney tissue earlier and to a greater extent. In contrast, the immersion group showed delayed immune responses with lower expression levels. The differential expression of 12 immune-related genes in immunized gibel carp, as detected by real-time quantitative PCR. Significant differences were observed in the expression of 12 immune-related genes after vaccination, suggesting variations in the sensitivity of each gene to bodily stimuli and its involvement in immunity. The specific immune mechanisms require further research. These results provide essential data for evaluating vaccines’ immune pathways and efficacy, offering optimism for vaccine development in fish immunology.

Abstract Image

CyHV-2减毒活疫苗对异育银鲫、异育银鲫免疫应答的影响
本研究对异育银鲫(Carassius auratus gibelio)浸泡和腹腔注射CyHV-2减毒活疫苗诱导的免疫应答进行了研究。在接种疫苗后3、7和14天对实验鱼的肾脏组织进行转录组测序分析。惊人的9.48亿个读数被组装成96,891个基因,平均长度为1214 bp。我们分析了浸泡组和注射组KEGG的富集程度、差异表达基因(DEGs)以及DEGs的时间表达模式。本研究结果显示,在异育银鲫体内注射和浸泡疫苗均能显著改变免疫相关基因,表明制备的减毒疫苗能有效刺激异育银鲫的免疫应答。一般来说,注射组在肾脏组织中表现出更高的基因表达水平,时间更早,程度更大。相比之下,浸泡组表现出延迟的免疫反应,表达水平较低。实时荧光定量PCR检测免疫后异育银鲫12个免疫相关基因的差异表达。接种疫苗后,12个免疫相关基因的表达有显著差异,表明每个基因对身体刺激的敏感性及其参与免疫的差异。具体的免疫机制有待进一步研究。这些结果为评估疫苗的免疫途径和功效提供了必要的数据,为鱼类免疫学疫苗的开发提供了乐观的前景。
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来源期刊
Aquaculture Research
Aquaculture Research 农林科学-渔业
CiteScore
4.60
自引率
5.00%
发文量
464
审稿时长
5.3 months
期刊介绍: International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.
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