Sensitive Assessment of Ketotifen Levels in Tablets and Plasma Using a Photoinduced Electron Transfer (PET) Inhibition Spectrofluorimetric Approach

IF 3.2 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2025-02-27 DOI:10.1002/bio.70134

Islam M. Mostafa, Abobakr A. Mohamed, Yaser Alahmadi, Ahmed K. B. Aljohani, Raed Alghamdi, Mahmoud A. Omar

{"title":"Sensitive Assessment of Ketotifen Levels in Tablets and Plasma Using a Photoinduced Electron Transfer (PET) Inhibition Spectrofluorimetric Approach","authors":"Islam M. Mostafa, Abobakr A. Mohamed, Yaser Alahmadi, Ahmed K. B. Aljohani, Raed Alghamdi, Mahmoud A. Omar","doi":"10.1002/bio.70134","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Ketotifen (KTF) is a type of asthma medication that is taken along with other antiasthma medications and may reduce the frequency, severity, and duration of asthma symptoms or attacks in children. This research introduces a novel spectrofluorimetric method for detecting KTF in its tablets and human blood plasma samples (spiked with KTF). The detection mechanism is based on a photoinduced electron transfer (PET) inhibition spectrofluorimetric approach for the sensitive detection of KTF different samples. By protonating KTF with 2.0 M acetic acid, the PET process is effectively inhibited, leading to a significant enhancement in the fluorescence intensity of KTF at 392 nm. This method detects KTF at values from 50 to 500 ng mL<sup>−1</sup>. The technique offers excellent detection and quantification limits of 13.41 and 40.65 ng mL<sup>−1</sup>, respectively. The range of the obtained percentage recoveries of KTF for spiked plasma samples was found to be 98.40% ± 1.30%–99.82% ± 1.20%, demonstrating the method's precision and reliability in diverse biological matrices. Also, the approach successfully evaluated KTF content uniformity in tablets without interference.</p>\n </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 3","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Luminescence","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bio.70134","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Ketotifen (KTF) is a type of asthma medication that is taken along with other antiasthma medications and may reduce the frequency, severity, and duration of asthma symptoms or attacks in children. This research introduces a novel spectrofluorimetric method for detecting KTF in its tablets and human blood plasma samples (spiked with KTF). The detection mechanism is based on a photoinduced electron transfer (PET) inhibition spectrofluorimetric approach for the sensitive detection of KTF different samples. By protonating KTF with 2.0 M acetic acid, the PET process is effectively inhibited, leading to a significant enhancement in the fluorescence intensity of KTF at 392 nm. This method detects KTF at values from 50 to 500 ng mL⁻¹. The technique offers excellent detection and quantification limits of 13.41 and 40.65 ng mL⁻¹, respectively. The range of the obtained percentage recoveries of KTF for spiked plasma samples was found to be 98.40% ± 1.30%–99.82% ± 1.20%, demonstrating the method's precision and reliability in diverse biological matrices. Also, the approach successfully evaluated KTF content uniformity in tablets without interference.

Abstract Image

查看原文本刊更多论文

利用光诱导电子转移（PET）抑制光谱荧光法灵敏评估片和血浆中酮替芬水平

酮替芬（KTF）是一种哮喘药物，与其他抗哮喘药物一起服用，可以降低儿童哮喘症状或发作的频率、严重程度和持续时间。本研究介绍了一种新的荧光光谱法检测其片剂和人血浆样品（加标KTF）中的KTF。检测机制是基于光诱导电子转移（PET）抑制光谱荧光法灵敏检测不同样品的KTF。用2.0 M乙酸将KTF质子化后，PET过程被有效抑制，导致KTF在392 nm处的荧光强度显著增强。该方法检测的KTF值从50到500 ng mL−1。该技术具有优良的检测限和定量限，分别为13.41和40.65 ng mL−1。加标血浆样品中KTF的回收率为98.40%±1.30% ~ 99.82%±1.20%，证明了该方法在不同生物基质中的精密度和可靠性。该方法在无干扰的情况下成功评价了片剂中KTF的含量均匀性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.