Rapid Biodegradation Assessment of Biomass Plastics Using Closed Recirculating Aquaculture Systems: A Novel Approach for Environmental Sustainability

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Wilasinee Kotcharoen, Naoki Wada, Makiko Kakikawa, Noboru Takiguchi, Yoshinori Takahashi, Kenji Takahashi, Yutaka Takeuchi
{"title":"Rapid Biodegradation Assessment of Biomass Plastics Using Closed Recirculating Aquaculture Systems: A Novel Approach for Environmental Sustainability","authors":"Wilasinee Kotcharoen,&nbsp;Naoki Wada,&nbsp;Makiko Kakikawa,&nbsp;Noboru Takiguchi,&nbsp;Yoshinori Takahashi,&nbsp;Kenji Takahashi,&nbsp;Yutaka Takeuchi","doi":"10.1007/s10126-025-10436-x","DOIUrl":null,"url":null,"abstract":"<div><p>The increasing plastic production causes serious problems in the marine environment, and the main source of plastic waste comes from the fishing and aquaculture industries. Although there have been various efforts to develop aquaculture equipment with marine biodegradable plastics, an urgent need is to develop an assay to evaluate their biodegradation in aquaculture environments. This study focused on evaluating the biodegradation of biomass plastic in recirculating aquaculture systems (RAS) that mimic freshwater, brackish water, and saltwater aquacultures. The methods used to assess biomass plastic biodegradability included changes in physical properties, weight loss, biochemical oxygen demand, and microbial community investigation using poly(butylene succinate-co-adipate) (PBSA) as a model. Scanning electron microscopy studies indicated the erosion on the biomass plastic surface from 1 to 2 days in the RAS tank (salinity, 0–0.5%) harboring Nile tilapia (<i>Oreochromis niloticus</i>). 4′,6-Diamidino-2-phenylindole fluorescence microscopy confirmed the presence of the microorganisms on the PBSA surface. The microorganisms in RAS tanks degraded 11.6% of 1 g/L PBSA in 7 days, demonstrating their biodegradation potential. 16S rRNA gene sequencing showed that <i>Pseudomonas</i> plays a major role as an early decomposer in the biodegradation process within 24 h. A multifaceted analytical method that provides sufficient evidence was developed to show that the erosion on the PBSA surface in RAS tanks results from biodegradation. The ability of RAS to control various aquatic environments (pH, salinity, temperature, and bacterial density) makes it suitable for testing the marine biodegradability of biomass plastics for use in aquaculture and fishery industries.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10436-x.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Biotechnology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10126-025-10436-x","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The increasing plastic production causes serious problems in the marine environment, and the main source of plastic waste comes from the fishing and aquaculture industries. Although there have been various efforts to develop aquaculture equipment with marine biodegradable plastics, an urgent need is to develop an assay to evaluate their biodegradation in aquaculture environments. This study focused on evaluating the biodegradation of biomass plastic in recirculating aquaculture systems (RAS) that mimic freshwater, brackish water, and saltwater aquacultures. The methods used to assess biomass plastic biodegradability included changes in physical properties, weight loss, biochemical oxygen demand, and microbial community investigation using poly(butylene succinate-co-adipate) (PBSA) as a model. Scanning electron microscopy studies indicated the erosion on the biomass plastic surface from 1 to 2 days in the RAS tank (salinity, 0–0.5%) harboring Nile tilapia (Oreochromis niloticus). 4′,6-Diamidino-2-phenylindole fluorescence microscopy confirmed the presence of the microorganisms on the PBSA surface. The microorganisms in RAS tanks degraded 11.6% of 1 g/L PBSA in 7 days, demonstrating their biodegradation potential. 16S rRNA gene sequencing showed that Pseudomonas plays a major role as an early decomposer in the biodegradation process within 24 h. A multifaceted analytical method that provides sufficient evidence was developed to show that the erosion on the PBSA surface in RAS tanks results from biodegradation. The ability of RAS to control various aquatic environments (pH, salinity, temperature, and bacterial density) makes it suitable for testing the marine biodegradability of biomass plastics for use in aquaculture and fishery industries.

利用封闭式循环水养殖系统对生物质塑料进行快速生物降解评估:实现环境可持续性的新方法
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信