Rapid Biodegradation Assessment of Biomass Plastics Using Closed Recirculating Aquaculture Systems: A Novel Approach for Environmental Sustainability

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Wilasinee Kotcharoen, Naoki Wada, Makiko Kakikawa, Noboru Takiguchi, Yoshinori Takahashi, Kenji Takahashi, Yutaka Takeuchi
{"title":"Rapid Biodegradation Assessment of Biomass Plastics Using Closed Recirculating Aquaculture Systems: A Novel Approach for Environmental Sustainability","authors":"Wilasinee Kotcharoen,&nbsp;Naoki Wada,&nbsp;Makiko Kakikawa,&nbsp;Noboru Takiguchi,&nbsp;Yoshinori Takahashi,&nbsp;Kenji Takahashi,&nbsp;Yutaka Takeuchi","doi":"10.1007/s10126-025-10436-x","DOIUrl":null,"url":null,"abstract":"<div><p>The increasing plastic production causes serious problems in the marine environment, and the main source of plastic waste comes from the fishing and aquaculture industries. Although there have been various efforts to develop aquaculture equipment with marine biodegradable plastics, an urgent need is to develop an assay to evaluate their biodegradation in aquaculture environments. This study focused on evaluating the biodegradation of biomass plastic in recirculating aquaculture systems (RAS) that mimic freshwater, brackish water, and saltwater aquacultures. The methods used to assess biomass plastic biodegradability included changes in physical properties, weight loss, biochemical oxygen demand, and microbial community investigation using poly(butylene succinate-co-adipate) (PBSA) as a model. Scanning electron microscopy studies indicated the erosion on the biomass plastic surface from 1 to 2 days in the RAS tank (salinity, 0–0.5%) harboring Nile tilapia (<i>Oreochromis niloticus</i>). 4′,6-Diamidino-2-phenylindole fluorescence microscopy confirmed the presence of the microorganisms on the PBSA surface. The microorganisms in RAS tanks degraded 11.6% of 1 g/L PBSA in 7 days, demonstrating their biodegradation potential. 16S rRNA gene sequencing showed that <i>Pseudomonas</i> plays a major role as an early decomposer in the biodegradation process within 24 h. A multifaceted analytical method that provides sufficient evidence was developed to show that the erosion on the PBSA surface in RAS tanks results from biodegradation. The ability of RAS to control various aquatic environments (pH, salinity, temperature, and bacterial density) makes it suitable for testing the marine biodegradability of biomass plastics for use in aquaculture and fishery industries.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 2","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10126-025-10436-x.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Biotechnology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10126-025-10436-x","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The increasing plastic production causes serious problems in the marine environment, and the main source of plastic waste comes from the fishing and aquaculture industries. Although there have been various efforts to develop aquaculture equipment with marine biodegradable plastics, an urgent need is to develop an assay to evaluate their biodegradation in aquaculture environments. This study focused on evaluating the biodegradation of biomass plastic in recirculating aquaculture systems (RAS) that mimic freshwater, brackish water, and saltwater aquacultures. The methods used to assess biomass plastic biodegradability included changes in physical properties, weight loss, biochemical oxygen demand, and microbial community investigation using poly(butylene succinate-co-adipate) (PBSA) as a model. Scanning electron microscopy studies indicated the erosion on the biomass plastic surface from 1 to 2 days in the RAS tank (salinity, 0–0.5%) harboring Nile tilapia (Oreochromis niloticus). 4′,6-Diamidino-2-phenylindole fluorescence microscopy confirmed the presence of the microorganisms on the PBSA surface. The microorganisms in RAS tanks degraded 11.6% of 1 g/L PBSA in 7 days, demonstrating their biodegradation potential. 16S rRNA gene sequencing showed that Pseudomonas plays a major role as an early decomposer in the biodegradation process within 24 h. A multifaceted analytical method that provides sufficient evidence was developed to show that the erosion on the PBSA surface in RAS tanks results from biodegradation. The ability of RAS to control various aquatic environments (pH, salinity, temperature, and bacterial density) makes it suitable for testing the marine biodegradability of biomass plastics for use in aquaculture and fishery industries.

利用封闭式循环水养殖系统对生物质塑料进行快速生物降解评估:实现环境可持续性的新方法
不断增加的塑料产量给海洋环境带来了严重的问题,而塑料废物的主要来源是渔业和水产养殖业。虽然已经有各种努力开发水产养殖设备与海洋生物可降解塑料,一个迫切需要是开发一种测定方法,以评估其在水产养殖环境中的生物降解。本研究的重点是评估生物质塑料在模拟淡水、微咸水和咸水养殖的循环水养殖系统(RAS)中的生物降解。生物质塑料生物降解性评价方法包括物理性能、失重、生化需氧量的变化,以及以聚丁二酸-共己二酸丁二烯(PBSA)为模型的微生物群落调查。扫描电镜研究表明,在养殖尼罗罗非鱼(Oreochromis niloticus)的RAS池(盐度0-0.5%)中,1 ~ 2天生物质塑料表面受到侵蚀。4′,6-二氨基-2-苯基吲哚荧光显微镜证实了PBSA表面存在微生物。RAS池中的微生物在7天内降解了1g /L PBSA的11.6%,显示了其生物降解潜力。16S rRNA基因测序表明,假单胞菌在24小时内的生物降解过程中作为早期分解者发挥了主要作用。一种多方面的分析方法提供了充分的证据,表明RAS罐中PBSA表面的侵蚀是由生物降解引起的。RAS控制各种水生环境(pH值、盐度、温度和细菌密度)的能力使其适合于测试水产养殖和渔业中使用的生物质塑料的海洋生物降解性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信