Extraction of Sanguisorba officinalis L. polysaccharide by ultrasound-assisted extraction: structural characterization, antioxidant, hemostatic and immunological activity

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-02-25 DOI:10.1016/j.ultsonch.2025.107292

Shuang Jiang , Xiaotian Wu , Xuepeng Shi , Yuanqiu Mu , Li Zhang , Shulu Zhang , Lin Wei , Zheng Feng , Yinze Zhong , Xinhui Huang , Yeqing Xu , Shah Syed Faizan Ali , Zhaonan Xu , Xiaotong Wang , Chunli Gan , Zhibin Wang , Yanan Sun , Chunjuan Yang

{"title":"Extraction of Sanguisorba officinalis L. polysaccharide by ultrasound-assisted extraction: structural characterization, antioxidant, hemostatic and immunological activity","authors":"Shuang Jiang , Xiaotian Wu , Xuepeng Shi , Yuanqiu Mu , Li Zhang , Shulu Zhang , Lin Wei , Zheng Feng , Yinze Zhong , Xinhui Huang , Yeqing Xu , Shah Syed Faizan Ali , Zhaonan Xu , Xiaotong Wang , Chunli Gan , Zhibin Wang , Yanan Sun , Chunjuan Yang","doi":"10.1016/j.ultsonch.2025.107292","DOIUrl":null,"url":null,"abstract":"<div><div><em>Sanguisorba officinalis</em> L. (<em>S</em>. <em>officinalis</em>) has been widely distributed in various regions of China and holds significant potential for various applications. However, limited studies have focused on <em>S</em>. <em>officinalis</em> polysaccharides (SOPs) and their biological activities. Utilizing response surface methodology (RSM), the process of ultrasonic-assisted extraction of SOPs was optimized. The optimal conditions were 270.2 W, 42.4 mL/g, and 47.7 min. Through separation and purification, a neutral polysaccharide named SOUP-1 was obtained. SOUP-1 (17.1 kDa) mainly includes glucose and a few galactose, mannose, and arabinose. The main chain of SOUP-1 is primarily → 4)-α-D-Glcp-(1 → 4,6)-α-D-Glcp-(1 → substituted at C6 with branch chain → 6)-α-D-Glcp-(1 → . Furthermore, SOUP-1 showed antioxidant activity, including scavenging activities of hydroxyl radicals and DPPH. It also enhances macrophage phagocytic activity and modulates the expression of inflammatory cytokines such as TNF-α and IL-6. Additionally, this study first discovered the hemostatic activity of SOUP-1. This study provides theoretical support for the potential applications and further research of SOPs. It provides a basis for the development of SOPs as novel natural-source immunomodulators in the food and pharmaceutical industries.</div></div>","PeriodicalId":442,"journal":{"name":"Ultrasonics Sonochemistry","volume":"115 ","pages":"Article 107292"},"PeriodicalIF":8.7000,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ultrasonics Sonochemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1350417725000719","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ACOUSTICS","Score":null,"Total":0}

引用次数: 0

Abstract

Sanguisorba officinalis L. (S. officinalis) has been widely distributed in various regions of China and holds significant potential for various applications. However, limited studies have focused on S. officinalis polysaccharides (SOPs) and their biological activities. Utilizing response surface methodology (RSM), the process of ultrasonic-assisted extraction of SOPs was optimized. The optimal conditions were 270.2 W, 42.4 mL/g, and 47.7 min. Through separation and purification, a neutral polysaccharide named SOUP-1 was obtained. SOUP-1 (17.1 kDa) mainly includes glucose and a few galactose, mannose, and arabinose. The main chain of SOUP-1 is primarily → 4)-α-D-Glcp-(1 → 4,6)-α-D-Glcp-(1 → substituted at C6 with branch chain → 6)-α-D-Glcp-(1 → . Furthermore, SOUP-1 showed antioxidant activity, including scavenging activities of hydroxyl radicals and DPPH. It also enhances macrophage phagocytic activity and modulates the expression of inflammatory cytokines such as TNF-α and IL-6. Additionally, this study first discovered the hemostatic activity of SOUP-1. This study provides theoretical support for the potential applications and further research of SOPs. It provides a basis for the development of SOPs as novel natural-source immunomodulators in the food and pharmaceutical industries.

查看原文本刊更多论文

超声辅助提取地榆多糖：结构表征、抗氧化、止血和免疫活性

山血orba officinalis L. （S. officinalis）广泛分布在中国各个地区，具有重要的应用潜力。然而，目前对officinalis polysaccharides （SOPs）及其生物活性的研究较少。利用响应面法（RSM）对超声辅助提取标准sop的工艺进行了优化。最佳提取条件为270.2 W、42.4 mL/g、47.7 min。通过分离纯化得到中性多糖SOUP-1。汤-1 （17.1 kDa）主要含有葡萄糖和少量半乳糖、甘露糖和阿拉伯糖。汤-1的主链主要是→4)-α- d - glcp -(1→4,6)-α- d - glcp -(1→在C6被支链→6)-α- d - glcp -(1→取代。此外，SOUP-1还具有抗氧化活性，包括清除羟基自由基和DPPH的活性。它还能增强巨噬细胞的吞噬活性，调节炎症细胞因子如TNF-α和IL-6的表达。此外，本研究首次发现了SOUP-1的止血活性。本研究为标准操作程序的潜在应用和进一步研究提供了理论支持。它为标准操作程序作为食品和制药工业中新型天然来源的免疫调节剂的发展提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.