Cationic liposomes encapsulating IL-2 selectively induce apoptosis and significantly reduce the secretion of cytokines on M1-murine polarized macrophages

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2025-02-28 DOI:10.1016/j.cyto.2025.156903

C.A. Vargas-Ángeles , L. Trujillo-Cirilo , E. Sierra-Mondragón , R. Rangel-Corona , B. Weiss-Steider

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Abstract

Inflammatory diseases pose a global challenge due to the critical role of macrophages in their pathogenesis. This study evaluated the effects of cationic liposomes encapsulating IL-2 (LIP-IL-2) on murine peritoneal macrophages (MP) polarized towards M1 and M2 phenotypes. M1 MP (CD86⁺), which overexpressed IL-2Rα and CD14 receptors, underwent apoptosis following LIP-IL-2 treatment, whereas M2 MP (CD206⁺) were unaffected. Furthermore, LIP-IL-2 significantly reduced the secretion of pro-inflammatory cytokines, including IL-6, TNF-α, IFN-γ, and IL-12, exclusively in M1 macrophages. These findings suggest that LIP-IL-2 could serve as a promising therapeutic tool for chronic inflammatory diseases by selectively inducing apoptosis in pro-inflammatory M1 macrophages without affecting M2 ones, opening avenues for targeted therapies in diseases where chronic macrophage-mediated inflammation is a key factor.

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包封IL-2的阳离子脂质体选择性诱导m1 -小鼠极化巨噬细胞凋亡，显著降低细胞因子的分泌

炎症性疾病是一项全球性挑战，因为巨噬细胞在这些疾病的发病机制中起着至关重要的作用。本研究评估了包裹IL-2的阳离子脂质体（LIP-IL-2）对极化为M1和M2表型的小鼠腹腔巨噬细胞（MP）的影响。经 LIP-IL-2 处理后，过度表达 IL-2Rα 和 CD14 受体的 M1 MP（CD86+）会发生凋亡，而 M2 MP（CD206+）则不受影响。此外，LIP-IL-2 还能显著减少促炎细胞因子的分泌，包括 IL-6、TNF-α、IFN-γ 和 IL-12，且仅在 M1 巨噬细胞中存在。这些研究结果表明，LIP-IL-2 可选择性地诱导促炎性 M1 巨噬细胞凋亡，而不影响 M2 巨噬细胞，从而为慢性巨噬细胞介导的炎症是一个关键因素的疾病的靶向治疗开辟了道路，可作为慢性炎症性疾病的一种有前途的治疗工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.