SmGRAS5 acts as a positive regulator in GA-induced biosynthesis of tanshinones in Salvia miltiorrhiza hairy roots

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-02-24 DOI:10.1016/j.plantsci.2025.112440

Wenrui Li , Suyu Lin , Ruihong Wang , Chen Chen , Qiliang Huang , Feng Ling , Zongsuo Liang

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引用次数: 0

Abstract

GA is an important phytohormone that regulates root growth and secondary metabolism. GRAS family transcription factors (TFs) are the key regulators of GA signaling. Here, we found that SmGRAS5 was co-expressed in the root periderm with tanshinones in Salvia miltiorrhiza. Overexpression (OE) of SmGRAS5 increased tanshinones accumulation and upregulated the biosynthetic genes. Antisense expression (AE) of SmGRAS5 reduced tanshinones accumulation and downregulated the biosynthetic genes. Yeast one-hybrid (Y1H), dual-luciferase (Dual-LUC), and electrophoretic mobility shift assays (EMSA) showed that SmGRAS5 promoted tanshinones biosynthesis by directly binding to the GARE motif in the promoter of SmKSL1 to induce its expression. However, overexpressing SmGRAS5 reduced GA content through downregulating the biosynthetic genes and also reduced root biomass. GA treatment further increased tanshinones accumulation and restored the root growth inhibited by overexpressing SmGRAS5. SmGRAS5 could not directly bind to the GA biosynthetic genes. Transcriptome analysis revealed the potential functions of SmGRAS5 in regulating secondary metabolism. Taken together, SmGRAS5 is involved in the regulation of GA-promoted tanshinones biosynthesis by directly activating the expression of SmKSL1, which suggests that SmGRAS5 may be a potential target for further metabolic engineering of tanshinones biosynthesis in S. miltiorrhiza.

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.