RNA interference-based gene silencing of dicer-like 2 in Fusarium oxysporum f. sp. cubense tropical race 4 mitigates fusarium wilt disease in banana

IF 3.4 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocatalysis and agricultural biotechnology Pub Date : 2025-02-22 DOI:10.1016/j.bcab.2025.103541

Egoda Ambhagahage Epitawaththe Samitha Sawindri Jayasekara , Ganesan Vadamalai , Norsazilawati Binti Saad , Jin Hailing , Wong Mui-Yun

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引用次数: 0

Abstract

Banana Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is a severe threat to global banana production which drastically reduces yields. Currently, no effective control measures exist for this destructive pathogen. RNA interference (RNAi)-based exogenous application of double-stranded RNAs (dsRNAs) has emerged as an environmental-friendly plant protection strategy in recent years. This innovative approach combats plant pathogens by silencing their essential genes. The dicer-like 2 (DCL2) gene which is an important component in the RNAi pathway, was silenced using in vitro synthesized long dsRNAs. The efficacy of Foc TR4-DCL2 dsRNAs was evaluated in vitro to determine optimal concentration and duration against Foc TR4, and their potential to suppress disease progression in planta. Relative transcript abundance analysis revealed that DCL2 gene was partially silenced at a threshold concentration of 1000 ng/mL after 72 h of dsRNA treatment, compared to the control (water) in vitro. In planta studies demonstrated that disease progression in roots and rhizomes was significantly inhibited in plants treated with Foc TR4-DCL dsRNAs, indicating that RNAi-mediated gene silencing mechanism could be a promising method for controlling banana Fusarium wilt in the future.

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来源期刊

Biocatalysis and agricultural biotechnology Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

7.70

自引率

2.50%

发文量

308

审稿时长

48 days

期刊介绍： Biocatalysis and Agricultural Biotechnology is the official journal of the International Society of Biocatalysis and Agricultural Biotechnology (ISBAB). The journal publishes high quality articles especially in the science and technology of biocatalysis, bioprocesses, agricultural biotechnology, biomedical biotechnology, and, if appropriate, from other related areas of biotechnology. The journal will publish peer-reviewed basic and applied research papers, authoritative reviews, and feature articles. The scope of the journal encompasses the research, industrial, and commercial aspects of biotechnology, including the areas of: biocatalysis; bioprocesses; food and agriculture; genetic engineering; molecular biology; healthcare and pharmaceuticals; biofuels; genomics; nanotechnology; environment and biodiversity; and bioremediation.