HR9 cell-penetrating peptide: An effective delivery approach of gene editing vectors for tumor treatment

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-02-25 DOI:10.1016/j.genrep.2025.102177

Ali Anvar , Azam Bolhassani , Iman Salahshoorifar , Shiva Irani

{"title":"HR9 cell-penetrating peptide: An effective delivery approach of gene editing vectors for tumor treatment","authors":"Ali Anvar , Azam Bolhassani , Iman Salahshoorifar , Shiva Irani","doi":"10.1016/j.genrep.2025.102177","DOIUrl":null,"url":null,"abstract":"<div><div>HR9 cell-penetrating peptide is an arginine-rich peptide that can deliver various molecules into the cells. In this study, we assessed the efficacy of HR9 peptide in transporting the modified CRISPR vectors pSpCas9n (BB)-2A-GFP (PX461) and pSpCas9n (BB) (PX460) into HPV16 C3 tumor cells, and explored the impact of their treatment through tumor growth assay, and immunohistochemistry and staining techniques. Herein, the HPV16 E6 and E7 genes were targeted using CRISPR/Cas9 technology. Our findings showed improved transfection rates of HR9 peptide compared to the Lipofectamine 2000 transfection reagent for delivery of the CRISPR/Cas9 vectors into C3 tumor cells. Mice treated with PX460-E7 and PX460-E6 + PX460-E7 showed important therapeutic effects regarding tumor volume reduction and pathological analysis. Our study demonstrated the potent delivery of HR9/CRISPR/Cas9 nanoparticles into C3 tumor cells <em>in vitro</em> and <em>in vivo</em>, and also the high efficiency and specificity of CRISPR/Cas9-mediated genome editing in mice.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102177"},"PeriodicalIF":1.0000,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452014425000500","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

HR9 cell-penetrating peptide is an arginine-rich peptide that can deliver various molecules into the cells. In this study, we assessed the efficacy of HR9 peptide in transporting the modified CRISPR vectors pSpCas9n (BB)-2A-GFP (PX461) and pSpCas9n (BB) (PX460) into HPV16 C3 tumor cells, and explored the impact of their treatment through tumor growth assay, and immunohistochemistry and staining techniques. Herein, the HPV16 E6 and E7 genes were targeted using CRISPR/Cas9 technology. Our findings showed improved transfection rates of HR9 peptide compared to the Lipofectamine 2000 transfection reagent for delivery of the CRISPR/Cas9 vectors into C3 tumor cells. Mice treated with PX460-E7 and PX460-E6 + PX460-E7 showed important therapeutic effects regarding tumor volume reduction and pathological analysis. Our study demonstrated the potent delivery of HR9/CRISPR/Cas9 nanoparticles into C3 tumor cells in vitro and in vivo, and also the high efficiency and specificity of CRISPR/Cas9-mediated genome editing in mice.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.