Genome-wide characterization of DREB transcription factors in Medicago truncatula: Insights into their roles in development and abiotic stress response

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-02-21 DOI:10.1016/j.genrep.2025.102170

Loua Haddoudi , Mariem Ayadi , Sabrine Hdira , Mohsen Hanana , Irene Romero , Hatem Ben Jouira , Naceur Djébali , Ludidi Ndiko , Maria Teresa Sanchez Ballesta , Chedly Abdelly , Mounawer Badri

{"title":"Genome-wide characterization of DREB transcription factors in Medicago truncatula: Insights into their roles in development and abiotic stress response","authors":"Loua Haddoudi , Mariem Ayadi , Sabrine Hdira , Mohsen Hanana , Irene Romero , Hatem Ben Jouira , Naceur Djébali , Ludidi Ndiko , Maria Teresa Sanchez Ballesta , Chedly Abdelly , Mounawer Badri","doi":"10.1016/j.genrep.2025.102170","DOIUrl":null,"url":null,"abstract":"<div><div>Dehydration-responsive-element binding (DREB) proteins play a crucial role in drought, salt, and environmental stress tolerance. In this study, we identified and annotated fifty-four DREB genes from the Medicago truncatula genome. These genes were analyzed at the molecular level, focusing on gene classification, genomic organization, phylogeny, synteny, structural features, and expression profiles. Phylogenetic analysis revealed that MtDREB proteins are categorized into six subgroups (A1–A6), with highly conserved motif compositions among them. Expression profiling showed that MtDREB genes are differentially expressed in various plant organs and under abiotic stresses (cold, salinity, and dehydration), with 30 % exhibiting high expression during flowering and development. Data from RNA-seq and microarrays demonstrated that 76 % of MtDREB genes are differentially expressed under at least one stress condition, indicating their involvement in various signaling pathways activated by abiotic stresses. Notably, MtDREB05, primarily induced under osmotic stress, appears to be a promising candidate for improving abiotic stress tolerance. These findings will enhance our understanding of the DREB family and aid in functional validation of DREBgenes in M. truncatula and related forage species.</div></div>","PeriodicalId":12673,"journal":{"name":"Gene Reports","volume":"39 ","pages":"Article 102170"},"PeriodicalIF":1.0000,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452014425000433","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Dehydration-responsive-element binding (DREB) proteins play a crucial role in drought, salt, and environmental stress tolerance. In this study, we identified and annotated fifty-four DREB genes from the Medicago truncatula genome. These genes were analyzed at the molecular level, focusing on gene classification, genomic organization, phylogeny, synteny, structural features, and expression profiles. Phylogenetic analysis revealed that MtDREB proteins are categorized into six subgroups (A1–A6), with highly conserved motif compositions among them. Expression profiling showed that MtDREB genes are differentially expressed in various plant organs and under abiotic stresses (cold, salinity, and dehydration), with 30 % exhibiting high expression during flowering and development. Data from RNA-seq and microarrays demonstrated that 76 % of MtDREB genes are differentially expressed under at least one stress condition, indicating their involvement in various signaling pathways activated by abiotic stresses. Notably, MtDREB05, primarily induced under osmotic stress, appears to be a promising candidate for improving abiotic stress tolerance. These findings will enhance our understanding of the DREB family and aid in functional validation of DREBgenes in M. truncatula and related forage species.

查看原文本刊更多论文

长尾紫花苜蓿DREB转录因子的全基因组特征：它们在发育和非生物应激反应中的作用

脱水响应元件结合（DREB）蛋白在干旱、盐和环境胁迫耐受中起着至关重要的作用。在本研究中，我们从苜蓿基因组中鉴定和注释了54个DREB基因。在分子水平上对这些基因进行分析，重点是基因分类、基因组组织、系统发育、合成、结构特征和表达谱。系统发育分析表明，MtDREB蛋白可分为6个亚群（A1-A6），其中的基序组成高度保守。表达谱显示，MtDREB基因在不同植物器官和非生物胁迫（寒冷、盐度和脱水）下存在差异表达，其中30%在开花和发育期间高表达。来自RNA-seq和微阵列的数据表明，76%的MtDREB基因在至少一种胁迫条件下差异表达，表明它们参与了非生物胁迫激活的各种信号通路。值得注意的是，主要在渗透胁迫下诱导的MtDREB05似乎是提高非生物胁迫耐受性的有希望的候选者。这些发现将增强我们对DREB家族的认识，并有助于对短尾草及相关牧草中DREB基因的功能验证。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.