Transport mechanisms of pesticide mixtures impairing intestinal barrier function in mice

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2025-02-25 DOI:10.1016/j.pestbp.2025.106356

Ziqi Liu , Kaijie Dai , Zhiyong Cao , Shujie Wang , Shuyan Gui , Wenyuan Hu , Tingting Meng , Yuying Liu , Jinjing Xiao , Haiqun Cao

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Abstract

Multiple pesticide residues in the diet can be ingested through the intestine; however, the interactions affecting intestinal health remain unclear. Histopathological analysis of mouse intestines revealed synergistic damage in those exposed to a binary mixture of abamectin, fluazinam, and spirodiclofen. The combined exposure to fluazinam and spirodiclofen resulted in a 73.35 % reduction in the expression level of the tight junction protein claudin-1 in Caco-2 cells. The studies on the transport in Caco-2 cells revealed that the combined exposure to abamectin and spirodiclofen resulted in transport amounts that were 5.37 and 19.98 times greater than those observed with individual exposures, respectively. The transporter inhibitors and molecular docking analysis indicated that competitive inhibition of the breast cancer resistance protein (BCRP) led to decreased pesticide efflux. Therefore, the disruption of the intestinal barrier caused by the interaction of pesticide mixtures warrants attention when evaluating the safety of various pesticide residues.

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.