Contribution of UDP-glycosyltransferases to clothianidin resistance in Bradysia odoriphaga

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2025-02-25 DOI:10.1016/j.pestbp.2025.106349

Chunni Zhang, Ruifang Liu, Xianglong Chen, Xianling Du, Shiying Tang, Wu Dai

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引用次数: 0

Abstract

Uridine diphosphate glycosyltransferases (UGTs) play critical roles in xenobiotic detoxification and are involved in insecticide resistance. In this study, UGT inhibitors, sulfinpyrazone and 5-nitrouracil, exhibited significant synergistic effects on clothianidin in the clothianidin-resistant strain (CL-R) of Bradysia odoriphaga. UGT enzyme content was significantly higher in the CL-R strain than in the susceptible strain (SS), and both the SS and CL-R strains showed a significant upregulation of UGT content after exposure to clothianidin. Two UGT genes, UGT36M1 and UGT306K1, were significantly overexpressed in the CL-R strain. UGT36M1 was predominantly expressed in the fat body, and UGT306K1 exhibited high levels of expression in the Malpighian tubules and midgut. UGT36M1 and UGT306K1 in the SS strain were significantly upregulated in response to clothianidin exposure. The silencing of UGT36M1 and UGT306K1 significantly enhanced the susceptibility of B. odoriphaga larvae to clothianidin. Furthermore, transgenic overexpression of UGT36M1 and UGT306K1 in Drosophila melanogaster significantly increased the tolerance of fruit flies to clothianidin. These findings provide evidence of the crucial role of UGT36M1 and UGT306K1 in conferring resistance to clothianidin in B. odoriphaga.

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.