A dual light-controlled co-culture system enables the regulation of population composition

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Synthetic and Systems Biotechnology Pub Date : 2025-02-19 DOI:10.1016/j.synbio.2025.02.012

Wei Jiang , Yijian Guo , Xuanshuo Liang , Ying Zhang , Jianning Kang , Zhengxin Jin , Bin Ning

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引用次数: 0

Abstract

With the development of metabolic engineering, increasing requirements for efficient microbial biosynthesis call for establishment of multi-strain co-culture system. Dynamic regulation of population ratios is crucial for optimizing bioproduction performance. Optogenetic systems with high universality and flexibility have the potential to realize dynamic control of population proportion. In this study, we utilized an optimized chromatic acclimation sensor/regulator (CcaS/R) system and a blue light-activated YF1-FixJ-PhlF system as induction modules. A pair of orthogonal quorum sensing systems and a toxin-antitoxin system were employed as communication module and effector module, respectively. By integrating these modules, we developed a dual light-controlled co-culture system that enables dynamic regulation of population ratios. This co-culture system provides a universal toolkit for applications in metabolic engineering and synthetic biology.

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双光控共培养系统可以调节种群组成

随着代谢工程技术的发展，对微生物高效合成的要求越来越高，需要建立多菌种共培养体系。种群比例的动态调节对优化生物生产性能至关重要。光遗传系统具有较高的通用性和灵活性，具有实现种群比例动态控制的潜力。在这项研究中，我们使用了一个优化的色驯化传感器/调节器（CcaS/R）系统和一个蓝光激活的YF1-FixJ-PhlF系统作为感应模块。以一对正交群体感应系统和一个毒素-抗毒素系统分别作为通信模块和效应模块。通过整合这些模块，我们开发了一个双光控共培养系统，可以动态调节种群比例。这种共培养系统为代谢工程和合成生物学的应用提供了一个通用的工具包。

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来源期刊

Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

6.90

自引率

12.50%

发文量

审稿时长

67 days

期刊介绍： Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.